Anti-Connexin 43 antibody

[STJ92411] Download PDF Print Data Sheet

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Product name Anti-Connexin 43 antibody
Short Description Rabbit polyclonal against Connexin 43
Description Connexin 43 is a protein encoded by the GJA1 gene which is approximately 43 kDa. Connexin 43 is localised to the cell membrane and endoplasmic reticulum. It is involved in gap junction trafficking and transport of connexins along the secretory pathway. It is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. In the heart these gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is expressed in the heart and foetal cochlea. Mutations in the GJA1 gene may result in Oculodentodigital dysplasia. STJ92411 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Connexin 43 protein.
Applications ELISA, IF, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Specificity Connexin 43 Polyclonal Antibody detects endogenous levels of Connexin 43 protein.
Protein Name Gap junction alpha-1 protein
Gap junction 43 kDa heart protein
Immunogen Synthesized peptide derived from human Connexin 43
Immunogen Region 310-390 aa, C-terminal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IF IHC WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 2697
Gene Symbol GJA1
Molecular Weight 43 kDa
Database Links HGNC:4274
Alternative Names Gap junction alpha-1 protein
Gap junction 43 kDa heart protein
Function Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract . May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles .
Post-translational Modifications Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity . Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation . Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2. S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.
Cellular Localization Cell membrane Cell junction, gap junction Endoplasmic reticulum. Localizes at the intercalated disk (ICD) in cardiomyocytes and the proper localization at ICD is dependent on TMEM65.
Tissue Specificity Expressed in the heart and fetal cochlea.
Swiss-Prot Key P17302_HUMAN
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