Anti-Cyclin B1 antibody

[STJ92534] Download PDF Print Data Sheet

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Product name Anti-Cyclin B1 antibody
Short Description Rabbit polyclonal to Cyclin B1.
Description Cyclin B1 is a protein encoded by the CCNB1 gene which is approximately 48,3 kDa. Cyclin B1 is localised to the cytoplasm and nucleus. It is involved in CDK-mediated phosphorylation and removal of Cdc6, regulation of PLK1 activity at G2/M transition and DNA damage response. This protein falls under the highly conserved cyclin family. It is a regulatory protein involved in mitosis. It forms a complex with p34(cdc2) to form the maturation-promoting factor. Cyclin B1 is expressed in the nervous system, skin, intestine, liver and lung. Mutations in the CCNB1 gene may result in thyroid lymphoma and cervical cancer. STJ92534 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Cyclin B1 protein.
Applications #N/A
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Specificity Cyclin B1 Polyclonal Antibody detects endogenous levels of Cyclin B1 protein.
Protein Name G2/mitotic-specific cyclin-B1
Immunogen Synthesized peptide derived from human Cyclin B1 around the non-phosphorylation site of S126.
Immunogen Region 60-140 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IF IHC WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 891
Gene Symbol CCNB1
Molecular Weight 60 kDa
Database Links HGNC:1579
Alternative Names G2/mitotic-specific cyclin-B1 antibody
CCNB antibody
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Post-translational Modifications Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
Cellular Localization Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Swiss-Prot Key P14635_HUMAN
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