Anti-E2F-1 (Acetyl Lys120) antibody

[STJ97800] Download PDF Print Data Sheet

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Product name Anti-E2F-1 (Acetyl Lys120) antibody
Short Description Rabbit polyclonal against E2F-1 (Acetyl Lys120)
Description E2F-1 is a protein encoded by the E2F1 gene which is approximately 46,9 kDa. E2F-1 is localised to the nucleus. It is involved in glioma, cyclins and cell cycle regulation and activation of BH3-only proteins. It plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. E2F-1 is expressed in the skin, pancreas and the nervous system. Mutations in the E2F1 gene may result in retinoblastoma pharyngoconjunctival fever. STJ97800 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous levels of E2F-1 (Acetyl Lys120).
Applications ELISA, WB
Dilution range WB 1:5000-10000 ELISA 1:10000
Specificity The antibody detects endogenous E2F-1 (Acetyl-Lys120) protein
Protein Name Transcription factor E2F1
Retinoblastoma-associated protein 1
Retinoblastoma-binding protein 3
pRB-binding protein E2F-1
Immunogen Synthetic peptide from AA range: 100-170.
Immunogen Region 100-170 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Isotype IgG
Formulation PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Gene ID 1869
Gene Symbol E2F1
Database Links HGNC:3113
Alternative Names Transcription factor E2F1
Retinoblastoma-associated protein 1
Retinoblastoma-binding protein 3
pRB-binding protein E2F-1
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters .
Post-translational Modifications Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis. Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
Cellular Localization Nucleus.
Swiss-Prot Key Q01094_HUMAN
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