Anti-Phospho-EF-2 (T56) antibody

[STJ91030] Download PDF Print Data Sheet

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Product name Anti-Phospho-EF-2 (T56) antibody
Short Description Rabbit polyclonal against Phospho-EF-2 (T56)
Description EF-2 is a protein encoded by the EEF2 gene which is approximately 95,3 kDa. EF-2 is localised to the cytoplasm and nucleus. It is involved in AMP-activated protein kinase signalling, viral mRNA translation, the innate immune system and the HIV life cycle. This protein falls under the GTP-binding translation elongation factor family. Its function is to catalyse the GTP dependent ribosomal translocation step during translation elongation. This involves the translocation of the nascent protein chain from the A-site to the P-site of the ribosome. EF-2 is expressed in the nervous system, liver, intestine, blood and lung. Mutations in the EEF2 gene may result in spinocerebellar ataxia and diphtheria. STJ91030 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous EF-2 protein which only binds about T56 when T56 is phosphorylated.
Applications ELISA, IF, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Specificity Phospho-EF-2 (T56) Polyclonal Antibody detects endogenous levels of EF-2 protein only when phosphorylated at T56.
Protein Name Elongation factor 2
Immunogen Synthesized peptide derived from human EF-2 around the phosphorylation site of T56.
Immunogen Region 1-80 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IHC WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1938
Gene Symbol EEF2
Molecular Weight 100 kDa
Database Links HGNC:3214
Alternative Names Elongation factor 2
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Post-translational Modifications Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment. Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis . ISGylated. Proteolytically processed at two sites following phosphorylation by CSK. SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage.
Cellular Localization Cytoplasm Nucleus. Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product.
Swiss-Prot Key P13639_HUMAN
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