Anti-Phospho-GABAB Receptor (Ser783) antibody (KLH)

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Product name Anti-Phospho-GABAB Receptor (Ser783) antibody (KLH)
Short Description Rabbit polyclonal against Phospho-GABAB Receptor (Ser783) (KLH)
Description Gamma-aminobutyric acid (GABA) was the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABAB receptors and phosphorylates S783 in the cytoplasmic tail of the R2 subunit and that S783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of S783 was evident in many brain regions and was increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007).
Applications IF, WB
Dilution range WB 1:1000; IF 1:500
Protein Name Gamma-aminobutyric acid type B receptor subunit 2
GABA-B receptor 2
G-protein coupled receptor 51
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser783 conjugated to KLH.
Storage Instruction Store at -20°C, and avoid repeated freeze-thaw cycles.
Note For research use only (RUO).
Validated Application WB IF
Host Rabbit
Clonality Polyclonal
Reactivity Avian, Bovine, Canine, Human, Simian, Xenopus
Conjugation KLH
Purification Anti-Phospho-GABAB Receptor (Ser783) antibody (KLH) was prepared using affinity purification from Pooled Serum
Formulation 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Gene ID 83633
Molecular Weight 102 kDa
Alternative Names Gamma-aminobutyric acid type B receptor subunit 2
GABA-B receptor 2
G-protein coupled receptor 51
Function Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
Cellular Localization Cell membrane. Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. Multi-pass membrane protein. Note: Coexpression of GABBR1 and GABBR2 was required for GABBR1 maturation and transport to the plasma membrane. In contrast, GABBR2 does not depend on GABBR1 for transport to the cell membrane.
Tissue Specificity Expressed in brain cortex, hippocampus, medial habenula, thalamus and cerebellum. Coexpression was seen in cerebellum.
Sequence and Domain Family Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.
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