Anti-Phospho-JAK1 (Y1022) antibody

[STJ90314] Download PDF Print Data Sheet

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Product name Anti-Phospho-JAK1 (Y1022) antibody
Short Description Rabbit polyclonal against Phospho-JAK1 (Y1022)
Description JAK1 is a protein encoded by the JAK1 gene which is approximately 133,2 kDa. JAK1 is localised to the endomembrane system. It is involved in RET signalling, Th17 cell differentiation, influenza A and IL27-mediated signalling events. It is a membrane protein that is a member of a class of protein-tyrosine kinases characterized by the presence of a second phosphotransferase-related domain at the N-terminal to the PTK domain. It plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. JAK1 is expressed in the nervous system, liver, blood, spleen and lung. Mutations in the JAK1 gene may result in chilblain lupus and T-cell prolymphocytic leukemia. STJ90314 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous JAK1 protein which only binds about Y1022 when Y1022 is phosphorylated.
Applications ELISA, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Specificity Phospho-JAK1 (Y1022) Polyclonal Antibody detects endogenous levels of JAK1 protein only when phosphorylated at Y1022.
Protein Name Tyrosine-protein kinase JAK1
Janus kinase 1
Immunogen Synthesized peptide derived from human JAK1 around the phosphorylation site of Y1022.
Immunogen Region 960-1040 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IF IHC WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 3716
Gene Symbol JAK1
Molecular Weight 132 kDa
Database Links HGNC:6190
Alternative Names Tyrosine-protein kinase JAK1
Janus kinase 1
Function Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway . Kinase partner for the interleukin (IL)-2 receptor .
Post-translational Modifications Autophosphorylated . Phosphorylated on tyrosine residues in response to interferon gamma signaling . Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling . Ubiquitinated by RNF125; leading to its degradation by the proteasome.
Cellular Localization Endomembrane system. Peripheral membrane protein. Wholly intracellular, possibly membrane associated.
Tissue Specificity Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
Sequence and Domain Family Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1. The FERM domain mediates interaction with JAKMIP1.
Swiss-Prot Key P23458_HUMAN
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