Anti-phospho-MLKL (S358) antibody

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Product name Anti-phospho-MLKL (S358) antibody
Short Description Mouse monoclonal against phospho-MLKL (S358)
Description MLKL is a protein encoded by the MLKL gene which is approximately 54,4 kDa. MLKL is localised to the cell membrane and cytoplasm. It is involved in the dimerization of procaspase-8, the TNF signalling pathway, apoptosis and autophagy. This protein falls under the protein kinase superfamily. It plays a critical role in tumour necrosis factor-induced necroptosis which is a programmed cell death process. It does this via interaction with the receptor-interacting protein 3 which is a key signalling molecule in necroptosis pathway. MLKL is expressed in the heart, intestine and lung. STJ97776 was developed from clone 6F8 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen MLKL. This primary antibody specifically binds to endogenous MLKL protein which only binds about S358 when S358 is phosphorylated.
Applications IHC
Dilution range IHC 1:100-200
Specificity phospho-MLKL (S358) Mouse Monoclonal Antibody (6F8) detects endogenous levels of MLKL
Protein Name Mixed lineage kinase domain-like protein
Immunogen synthetic peptide derived from phospho-MLKL (S358)
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IHC
Host Mouse
Clonality Monoclonal
Clone ID 6F8
Reactivity Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 197259
Gene Symbol MLKL
Database Links HGNC:26617
Alternative Names Mixed lineage kinase domain-like protein
Function Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.
Post-translational Modifications Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.
Cellular Localization Cytoplasm Cell membrane. Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.
Sequence and Domain Family The protein kinase domain is catalytically inactive but contains an unusual pseudoactive site with an interaction between Lys-230 and Gln-356 residues. Upon phosphorylation by RIPK3, undergoes an active conformation . The coiled coil region 2 is responsible for homotrimerization.
Swiss-Prot Key Q8NB16_HUMAN
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