Anti-PKM2 antibody

[STJ95142] Download PDF Print Data Sheet

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Product name Anti-PKM2 antibody
Short Description Rabbit polyclonal against PKM2
Description PKM2 is a protein encoded by the PKM gene which is approximately 57,9 kDa. PKM2 is localised to the cytoplasm and nucleus. It is involved in glucose metabolism, innate immune system and carbon metabolism and glucose / energy metabolism. It is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation and pays a general role in caspase independent cell death of tumor cells. PKM2 is specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. Mutations in the PKM gene may result in pyruvate kinase deficiency and gastric diffuse adenocarcinoma. STJ95142 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody binds to endogenous levels of PKM2 protein.
Applications ELISA, WB
Dilution range WB 1:500-1:2000
ELISA 1:10000
Specificity PKM2 Polyclonal Antibody detects endogenous levels of PKM2 protein.
Protein Name Pyruvate kinase PKM
Cytosolic thyroid hormone-binding protein
CTHBP
Opa-interacting protein 3
OIP-3
Pyruvate kinase 2/3
Pyruvate kinase muscle isozyme
Thyroid hormone-binding protein 1
THBP1
Tumor M2-P
Immunogen Synthesized peptide derived from human PKM2.
Immunogen Region Internal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IF IHC WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 5315
Gene Symbol PKM
Molecular Weight 58 kDa
Database Links HGNC:9021
OMIM:179050
Alternative Names Pyruvate kinase PKM
Cytosolic thyroid hormone-binding protein
CTHBP
Opa-interacting protein 3
OIP-3
Pyruvate kinase 2/3
Pyruvate kinase muscle isozyme
Thyroid hormone-binding protein 1
THBP1
Tumor M2-P
Function Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Post-translational Modifications ISGylated. Under hypoxia, hydroxylated by EGLN3. Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy. FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
Cellular Localization Cytoplasm Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
Tissue Specificity Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
Swiss-Prot Key P14618_HUMAN
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