Anti-Ub (Acetyl K48) antibody

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Product name Anti-Ub (Acetyl K48) antibody
Short Description Rabbit polyclonal against Ub (Acetyl K48)
Description Rabbit polyclonal to Acetyl-Ub (K48).
Applications ELISA, WB
Dilution range WB 1:500-1:2000
ELISA 1:20000
Specificity Acetyl-Ub (K48) Polyclonal AntibodySynthesized peptide derived from the human Ub around the acetylation site of K48.
Protein Name Ubiquitin-60S ribosomal protein L40
Ubiquitin A-52 residue ribosomal protein fusion product 1
60S ribosomal protein L40
Large ribosomal subunit protein eL40
Immunogen Synthesized peptide derived from the human Ub
Immunogen Region around the acetylation site of K48.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 7311
Gene Symbol UBA52
Database Links HGNC:12458
Alternative Names Ubiquitin-60S ribosomal protein L40
Ubiquitin A-52 residue ribosomal protein fusion product 1
60S ribosomal protein L40
Large ribosomal subunit protein eL40
Function Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.; 60S ribosomal protein L40: Component of the 60S subunit of the ribosome. Ribosomal protein L40 is essential for translation of a subset of cellular transcripts, and especially for cap-dependent translation of vesicular stomatitis virus mRNAs.
Post-translational Modifications Ubiquitin: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy . Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30 .
Cellular Localization Ubiquitin: Cytoplasm Nucleus 60S ribosomal protein L40: Cytoplasm
Swiss-Prot Key P62987_HUMAN
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