Anti-LC3A antibody

[STJ97755] Download PDF Print Data Sheet

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Product name Anti-LC3A antibody
Short Description Mouse monoclonal against LC3A
Description LC3A is a protein encoded by the MAP1LC3A gene which is approximately 14, 3 kD. LC3A is localised to the cytoplasm, cytoskeleton and autophagosome. It is involved in pathways such as the autophagy pathway, pink/parkin mediated mitophagy, ferroptosis, cellular senescence and pathways affecting insulin-like growth factor-Akt signalling. MAP1A and MAP1B fall under the LC3A protein. They are microtubule-associated proteins that mediate the physical interactions between microtubules and components of the cytoskeleton. LC3A is most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. Mutations in the LC3A gene may result in Nasu-Hakola disease, lacrimal gland adenocarcinoma and leber congenital amaurosis. STJ97755 was developed from clone 5G10. It was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous levels of LC3A.
Applications IF, IHC-p, WB
Dilution range WB 1:1000-2000
IHC 1:100-200
IF 1:200
Specificity LC3A protein detects endogenous levels of LC3A
Protein Name Microtubule-Associated Proteins 1a/1b Light Chain 3a
Autophagy-Related Protein Lc3 A
Autophagy-Related Ubiquitin-Like Modifier Lc3 A
Map1 Light Chain 3-Like Protein 1
Map1a/Map1b Light Chain 3 A
Map1a/Map1b Lc3 A
Microtubule-Associated Protein 1 Light Chain 3 Alpha
Immunogen synthetic peptide derived from LC3A
Storage Instruction Store at-20°C, and avoid repeat freeze-thaw cycles.
Note FOR RESEARCH USE ONLY (RUO).
Host Mouse
Clonality Monoclonal
Clone ID 5G10
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 84557
Gene Symbol MAP1LC3A
Database Links HGNC:6838
OMIM:601242
Alternative Names Anti-Microtubule-Associated Proteins 1a/1b Light Chain 3a antibody
Anti-Autophagy-Related Protein Lc3 A antibody
Anti-Autophagy-Related Ubiquitin-Like Modifier Lc3 A antibody
Anti-Map1 Light Chain 3-Like Protein 1 antibody
Anti-Map1a/Map1b Light Chain 3 A antibody
Anti-Map1a/Map1b Lc3 A antibody
Anti-Microtubule-Associated Protein 1 Light Chain 3 Alpha antibody
Anti-MAP1LC3A antibody
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, paticipates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover.
Post-translational Modifications The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.; The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.; Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.
Cellular Localization Cytoplasm
Cytoskeleton
Endomembrane System
Lipid-Anchor
Cytoplasmic Vesicle
Autophagosome Membrane
Autophagosome
Lc3-Ii Binds To The Autophagic Membranes
Tissue Specificity Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
Swiss-Prot Key MLP3A_HUMAN
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