Loading Control

 

St John's Laboratory offers a variety of primary polyclonal and monoclonal antibodies. With numerous cited primary and secondary antibodies available from our catalogue of more than 30,000 antibodies we have you covered for your western blot, immunofluorescence, ELISA and other assay needs. So whether you are after rabbit monoclonals, goat polyclonals or ELISA kits, we provide coverage for the majority of research areas.

 

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Featured Products

Feature Product - Anti-Beta Actin Antibody (STJ96930)

Feature Product - Anti-Beta Actin primary antibody (STJ96930)

Mouse monoclonal Anti-Beta Actin primary antibody with 4 reviews over 4-star, covering multiple applications and multiple species, at high dilution.

IHC Guaranteed Antibodies

IHC-Guarantee primary antibodies

We have selected a wide range of IHC-validated primary antibodies suitable for immunohistochemistry applications, covering pathology, cancer research, immunology and other usual biomarkers.

  Loading Controls

Loading controls

Loading control primary antibodies target proteins which typically exhibit ubiquitous expression. There use is valuable in determining relative protein concentration and in determining the successful transfer of protein in western blots.

Rabbit Monoclonals

Rabbit monoclonals

Primary rabbit monoclonal antibodies can exhibit a greater binding affinity to antigens than those from rodents such as mice. Using rabbit monoclonal antibodies is particularly advantageous for more accurate immunohistochemistry results.

Phospho Antibodies

Phospho-antibodies

Protein phosphorylation plays a significant role in a wide range of post-translational cellular processes including cell growth and proliferation, metabolism, physiological regulation and cell signalling.

Knockout

KO-validated antibodies

        In a knockout validation, antibody specificity is confirmed by testing the antibody of interest against a sample from a tissue or cell line which does not express the protein of interest as well as a positive control which does.

Goat Primary Antibodies

Goat primary antibodies

Goat primary antibodies are ideal for large scale projects at a low cost. We have over 3000 goat primary antibodies suitable for various applications.

Tag antibodies

Tag antibodies

Tag antibodies are frequently used with recombinant protein cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. One of the most popular is our Anti-mCherry-Tag antibody used widely in gene expression assays.

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  • Anti-Phospho-Paxillin (Y31) antibody [STJ90380]

    Western Blot (WB) analysis of specific cells using Phospho-Paxillin (Y31) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, WB
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  • Anti-Phospho-MYL12A-(S18) Antibody [STJ22304]

    Western blot analysis of extracts from 293 cells untreated or treated with starvation using Phospho-MYL12A-S18 antibody (STJ22304). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: IHC, WB
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  • Anti-Phospho-Caldesmon (S789) antibody [STJ91034]

    Western Blot (WB) analysis of specific cells using Phospho-Caldesmon (S789) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC, WB
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  • Anti-Phospho-CaMKI alpha/delta (T286) antibody [STJ90202]

    Western Blot (WB) analysis of HeLa cells using Phospho-CaMKIIalpha/delta (T286) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC, WB
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  • Anti-Phospho-IP3R-I (S1598) antibody [STJ91314]

    Immunohistochemical analysis of paraffin-embedded Human cervix cancer. Antibody was diluted at 1:100 (4°C, overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antibody retrieval. Negative control (right) obtained from antibody was pre-
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC
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  • Anti-Phospho-Calmodulin (T80/S82) antibody [STJ91072]

    Western Blot (WB) analysis of specific cells using Phospho-Calmodulin (T80/S82) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC, WB
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  • Anti-Phospho-CaMKI beta/gamma/delta (T287) antibody [STJ90941]

    Western blot (WB) analysis of CaMKIIbeta/gamma/delta (phospho Thr287) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, WB
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  • Anti-Phospho-Troponin I-C (S43) antibody [STJ90883]

    Immunohistochemical analysis of paraffin-embedded Human heart. Antibody was diluted at 1:100 (4°C, overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antibody retrieval. Negative control (right) obtained from antibody was pre-absorbed
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, WB
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  • Anti-Phospho-PA alpha (T212) antibody [STJ90826]

    Western Blot (WB) analysis of 293 cells using Phospho-PAKalpha (T212) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, WB
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  • Anti-Phospho-VIM-(S39) Antibody [STJ117904]

    Western blot analysis of extracts of Jurkat cells, using Phospho-VIM-S39 antibody (STJ117904) at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: WB
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  • Anti-Phospho-VIM-(S56) Antibody [STJ117902]

    Western blot analysis of extracts of HeLa cells, using Phospho-VIM-S56 antibody (STJ117902) at 1:3000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours or treated by Paclitaxel (100nM/ml) for 20 hours. Secondary antibody: HRP Goat Anti-R
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Rat
    Applications: WB
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  • Anti-Phospho-PAK2-(S20) Antibody [STJ117901]

    Western blot analysis of extracts of HeLa and NIH/3T3 cells, using Phospho-PAK2-S20 antibody (STJ117901) at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Calyc
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse
    Applications: WB
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