Loading Control

 

St John's Laboratory offers a variety of primary polyclonal and monoclonal antibodies. With numerous cited primary and secondary antibodies available from our catalogue of more than 30,000 antibodies we have you covered for your western blot, immunofluorescence, ELISA and other assay needs. So whether you are after rabbit monoclonals, goat polyclonals or ELISA kits, we provide coverage for the majority of research areas.

 

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Featured Products

Feature Product - Anti-Beta Actin Antibody (STJ96930)

Feature Product - Anti-Beta Actin primary antibody (STJ96930)

Mouse monoclonal Anti-Beta Actin primary antibody with 4 reviews over 4-star, covering multiple applications and multiple species, at high dilution.

IHC Guaranteed Antibodies

IHC-Guarantee primary antibodies

We have selected a wide range of IHC-validated primary antibodies suitable for immunohistochemistry applications, covering pathology, cancer research, immunology and other usual biomarkers.

  Loading Controls

Loading controls

Loading control primary antibodies target proteins which typically exhibit ubiquitous expression. There use is valuable in determining relative protein concentration and in determining the successful transfer of protein in western blots.

Rabbit Monoclonals

Rabbit monoclonals

Primary rabbit monoclonal antibodies can exhibit a greater binding affinity to antigens than those from rodents such as mice. Using rabbit monoclonal antibodies is particularly advantageous for more accurate immunohistochemistry results.

Phospho Antibodies

Phospho-antibodies

Protein phosphorylation plays a significant role in a wide range of post-translational cellular processes including cell growth and proliferation, metabolism, physiological regulation and cell signalling.

Knockout

KO-validated antibodies

        In a knockout validation, antibody specificity is confirmed by testing the antibody of interest against a sample from a tissue or cell line which does not express the protein of interest as well as a positive control which does.

Goat Primary Antibodies

Goat primary antibodies

Goat primary antibodies are ideal for large scale projects at a low cost. We have over 3000 goat primary antibodies suitable for various applications.

Tag antibodies

Tag antibodies

Tag antibodies are frequently used with recombinant protein cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. One of the most popular is our Anti-mCherry-Tag antibody used widely in gene expression assays.

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  • Anti-Phospho-ERK 1/2 (Y222/205) antibody [STJ90657]

    Western blot (WB) analysis of ERK 1/2 (phospho Tyr222/205) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, WB
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  • Anti-Phospho-Raf-1 (S259) antibody [STJ90399]

    Western Blot (WB) analysis of specific cells using Phospho-Raf-1 (S259) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat, Simian
    Applications: ELISA, IHC, WB
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  • Anti-Phospho-Trk C (Y516) antibody [STJ90960]

    Western Blot (WB) analysis of HeLa cells using Phospho-Trk C (Y516) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, FC, IHC, WB
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  • Anti-Phospho-Synapsin I (S9) antibody [STJ90417]

    Western Blot (WB) analysis of specific cells using Phospho-Synapsin I (S9) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC, WB
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  • Anti-Phospho-CREB-1 (S133) antibody [STJ90235]

    Western blot (WB) analysis of CREB-1 (phospho Ser133) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, IP, WB
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  • Anti-Phospho-CaMKI alpha/delta (T286) antibody [STJ90202]

    Western Blot (WB) analysis of HeLa cells using Phospho-CaMKIIalpha/delta (T286) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC, WB
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  • Anti-Phospho-NMD zeta (S890) antibody [STJ91295]

    Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C, overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antibody retrieval. Negative control (right) obtained from antibody was pre-absorbed
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC
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  • Anti-Phospho-Calmodulin (T80/S82) antibody [STJ91072]

    Western Blot (WB) analysis of specific cells using Phospho-Calmodulin (T80/S82) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC, WB
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  • Anti-Phospho-CaMKI beta/gamma/delta (T287) antibody [STJ90941]

    Western blot (WB) analysis of CaMKIIbeta/gamma/delta (phospho Thr287) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, WB
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  • Anti-Phospho-GRIN1-(S890) Antibody [STJ117924]

    Western blot analysis of extracts of C6 cells, using Phospho-GRIN1-S890 antibody (STJ117924) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) a
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Rat
    Applications: WB
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  • Anti-Phospho-GRIA1-(S845) Antibody [STJ117923]

    Western blot analysis of extracts of SH-SY5Y cells, using Phospho-GRIA1-S845 antibody (STJ117923) at 1:1000 dilution. SH-SY5Y cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour . Secondary antibody: HRP Goat Anti-Rabbi
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: WB
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  • Anti-Phospho-PRKCA-(S319) Antibody [STJ117877]

    Western blot analysis of extracts of 293T cells, using Phospho-PKCalpha-S319 antibody (STJ117877). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human
    Applications: WB
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