Loading Control

 

St John's Laboratory offers a variety of primary polyclonal and monoclonal antibodies. With numerous cited primary and secondary antibodies available from our catalogue of more than 30,000 antibodies we have you covered for your western blot, immunofluorescence, ELISA and other assay needs. So whether you are after rabbit monoclonals, goat polyclonals or ELISA kits, we provide coverage for the majority of research areas.

 

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Featured Products

Feature Product - Anti-Beta Actin Antibody (STJ96930)

Feature Product - Anti-Beta Actin primary antibody (STJ96930)

Mouse monoclonal Anti-Beta Actin primary antibody with 4 reviews over 4-star, covering multiple applications and multiple species, at high dilution.

IHC Guaranteed Antibodies

IHC-Guarantee primary antibodies

We have selected a wide range of IHC-validated primary antibodies suitable for immunohistochemistry applications, covering pathology, cancer research, immunology and other usual biomarkers.

  Loading Controls

Loading controls

Loading control primary antibodies target proteins which typically exhibit ubiquitous expression. There use is valuable in determining relative protein concentration and in determining the successful transfer of protein in western blots.

Rabbit Monoclonals

Rabbit monoclonals

Primary rabbit monoclonal antibodies can exhibit a greater binding affinity to antigens than those from rodents such as mice. Using rabbit monoclonal antibodies is particularly advantageous for more accurate immunohistochemistry results.

Phospho Antibodies

Phospho-antibodies

Protein phosphorylation plays a significant role in a wide range of post-translational cellular processes including cell growth and proliferation, metabolism, physiological regulation and cell signalling.

Knockout

KO-validated antibodies

        In a knockout validation, antibody specificity is confirmed by testing the antibody of interest against a sample from a tissue or cell line which does not express the protein of interest as well as a positive control which does.

Goat Primary Antibodies

Goat primary antibodies

Goat primary antibodies are ideal for large scale projects at a low cost. We have over 3000 goat primary antibodies suitable for various applications.

Tag antibodies

Tag antibodies

Tag antibodies are frequently used with recombinant protein cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. One of the most popular is our Anti-mCherry-Tag antibody used widely in gene expression assays.

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Product Type: Rabbit Monoclonals
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  • Anti-Phospho-RB1-(S807) Antibody [STJ113523]

    Western blot analysis of extracts of A549 cells, using Phospho-RB1-S807 antibody (STJ113523). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
    Clonality: Monoclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: IF, IHC, WB
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  • Anti-MCM3 antibody [STJ180165]

    Formalin-fixed, paraffin-embedded human normal colon mucosa stained with anti-MCM3 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells
    Clonality: Monoclonal
    Host: Rabbit
    Reactivity: Human
    Applications: IHC
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  • Anti-MYC antibody [STJ180021]

    Formalin-fixed, paraffin-embedded human Burkitt lymphoma stained with anti-c-myc antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells
    Clonality: Monoclonal
    Host: Rabbit
    Reactivity: Human
    Applications: IHC
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  • Anti-CCND1 antibody [STJ180082]

    Formalin-fixed, paraffin-embedded human mantle cell lymphoma stained with anti-Cyclin D1 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of lymphoma cells
    Clonality: Monoclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: IHC
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  • Anti-AcetylH4K5AC-(K5) Antibody [STJ117427]

    Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (STJ117427) at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours. Secondary antibody: HRP Goat A
    Clonality: Monoclonal
    Host: Rabbit
    Reactivity: Human, Rat
    Applications: WB
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  • Anti-TUBB Antibody [STJ117189]

    Western blot analysis of extracts of various cell lines, using TUBB antibody (STJ117189). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
    Clonality: Monoclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: IF, IHC, WB
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  • Anti-H2AFX antibody [STJ113824]

    Western blot analysis of extracts of various cell lines, using H2AFX antibody (STJ113824) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in T
    Clonality: Monoclonal
    Host: Rabbit
    Reactivity: Human, Mouse
    Applications: WB
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  • Anti-AcetylH2A K9ac-(K9) Antibody [STJ116415]

    Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K9 antibody (STJ116415). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST
    Clonality: Monoclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: IF, IHC, WB
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  • Anti-Phospho-Histone H3-S10 antibody [STJ130027]

    Immunofluorescence analysis of HepG2, HeLa and AGS cells using rabbit monoclonal Phospho-Histone H3-S10 antibody, using Anti-Phopsho-Histone H3-S10 monoclonal antibody (STJ130027).
    Clonality: Monoclonal
    Host: Rabbit
    Reactivity: Human
    Applications: FC, IF, IHC, WB
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  • Anti-AcetylH4K16ac-(K16) Antibody [STJ116277]

    Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K16 antibody (STJ116277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonf
    Clonality: Monoclonal
    Host: Rabbit
    Reactivity: Human, Mouse
    Applications: WB
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  • Anti-CCND3 Antibody [STJ114943]

    Western blot analysis of extracts of various cell lines, using CCND3 antibody (STJ114943) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in T
    Clonality: Monoclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: WB
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  • Anti-CDKN1A Antibody [STJ114923]

    Western blot analysis of extracts of various cell lines, using CDKN1A antibody (STJ114923) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in
    Clonality: Monoclonal
    Host: Rabbit
    Reactivity: Human
    Applications: IF, IHC, IP, WB
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