Loading Control

 

St John's Laboratory offers a variety of primary polyclonal and monoclonal antibodies. With numerous cited primary and secondary antibodies available from our catalogue of more than 30,000 antibodies we have you covered for your western blot, immunofluorescence, ELISA and other assay needs. So whether you are after rabbit monoclonals, goat polyclonals or ELISA kits, we provide coverage for the majority of research areas.

 

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Featured Products

Feature Product - Anti-Beta Actin Antibody (STJ96930)

Feature Product - Anti-Beta Actin primary antibody (STJ96930)

Mouse monoclonal Anti-Beta Actin primary antibody with 4 reviews over 4-star, covering multiple applications and multiple species, at high dilution.

IHC Guaranteed Antibodies

IHC-Guarantee primary antibodies

We have selected a wide range of IHC-validated primary antibodies suitable for immunohistochemistry applications, covering pathology, cancer research, immunology and other usual biomarkers.

  Loading Controls

Loading controls

Loading control primary antibodies target proteins which typically exhibit ubiquitous expression. There use is valuable in determining relative protein concentration and in determining the successful transfer of protein in western blots.

Rabbit Monoclonals

Rabbit monoclonals

Primary rabbit monoclonal antibodies can exhibit a greater binding affinity to antigens than those from rodents such as mice. Using rabbit monoclonal antibodies is particularly advantageous for more accurate immunohistochemistry results.

Phospho Antibodies

Phospho-antibodies

Protein phosphorylation plays a significant role in a wide range of post-translational cellular processes including cell growth and proliferation, metabolism, physiological regulation and cell signalling.

Knockout

KO-validated antibodies

        In a knockout validation, antibody specificity is confirmed by testing the antibody of interest against a sample from a tissue or cell line which does not express the protein of interest as well as a positive control which does.

Goat Primary Antibodies

Goat primary antibodies

Goat primary antibodies are ideal for large scale projects at a low cost. We have over 3000 goat primary antibodies suitable for various applications.

Tag antibodies

Tag antibodies

Tag antibodies are frequently used with recombinant protein cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. One of the most popular is our Anti-mCherry-Tag antibody used widely in gene expression assays.

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  • Anti-Phospho-Raf-1 (S259) antibody [STJ90399]

    Western Blot (WB) analysis of specific cells using Phospho-Raf-1 (S259) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat, Simian
    Applications: ELISA, IHC, WB
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  • Anti-Phospho-eIF alpha (S51) antibody [STJ90253]

    Western blot (WB) analysis of eIF2alpha (phospho Ser51) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, WB
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  • Anti-phospho-MLKL (S358) antibody [STJ97776]

    Immunohistochemistry (IHC) analysis of paraffin-embedded Human Skin Tissue using Phospho-MLKL S358 Mouse monoclonal antibody diluted at 1:200.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Human
    Applications: IHC
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  • Anti-Phospho-SGK1 (S422) antibody [STJ90404]

    Western Blot (WB) analysis of VEC HY926 cells using Phospho-SGK1 (S422) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, WB
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  • Anti-Phospho-PKA I beta reg (S113) antibody [STJ90520]

    Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C, overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antibody retrieval. Negative control (right) obtained from antibody was pre-a
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat, Simian
    Applications: ELISA, IHC, WB
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  • Anti-Phospho-CaMKI alpha/delta (T286) antibody [STJ90202]

    Western Blot (WB) analysis of HeLa cells using Phospho-CaMKIIalpha/delta (T286) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC, WB
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  • Anti-Phospho-Calmodulin (T80/S82) antibody [STJ91072]

    Western Blot (WB) analysis of specific cells using Phospho-Calmodulin (T80/S82) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC, WB
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  • Anti-Phospho-CaMKI beta/gamma/delta (T287) antibody [STJ90941]

    Western blot (WB) analysis of CaMKIIbeta/gamma/delta (phospho Thr287) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, WB
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  • Anti-Phospho-WNK1 (T60) antibody [STJ90776]

    Western Blot (WB) analysis of specific cells using Phospho-WNK1 (T60) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, WB
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  • Anti-Phospho-AP2M1-(T156) Antibody [STJ117921]

    Western blot analysis of extracts of 293T and NIH/3T3 cells, using Phospho-AP2M1-T156 antibody (STJ117921) at 1:1000 dilution. 293T cells were treated by Calyculin A for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Hydrogen Pe
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: WB
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  • Anti-Phospho-Dopamine Transporter (Thr53) antibody (KLH) [STJ120056]

    Western blot of rat striatal lysate showing specific immunolabeling of the ~55 kDa glycosylated form of the DAT protein phosphorylated at Thr53 in the first lane (-) Phosphospecificity was shown in the second lane (+) where immunolabeling was blocked by p
    Clonality: Polyclonal
    Host: Rabbit
    Applications: IHC, WB
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  • Anti-Phospho-EIF2S1-(S51) Antibody [STJ116376]

    Western blot analysis of extracts of HeLa and Jurkat cells,using Phospho-eIF2α-S51 antibody (STJ116376) at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calycu
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human
    Applications: IP, WB
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