Loading Control

 

St John's Laboratory offers a variety of primary polyclonal and monoclonal antibodies. With numerous cited primary and secondary antibodies available from our catalogue of more than 30,000 antibodies we have you covered for your western blot, immunofluorescence, ELISA and other assay needs. So whether you are after rabbit monoclonals, goat polyclonals or ELISA kits, we provide coverage for the majority of research areas.

 

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Featured Products

Feature Product - Anti-Beta Actin Antibody (STJ96930)

Feature Product - Anti-Beta Actin primary antibody (STJ96930)

Mouse monoclonal Anti-Beta Actin primary antibody with 4 reviews over 4-star, covering multiple applications and multiple species, at high dilution.

IHC Guaranteed Antibodies

IHC-Guarantee primary antibodies

We have selected a wide range of IHC-validated primary antibodies suitable for immunohistochemistry applications, covering pathology, cancer research, immunology and other usual biomarkers.

  Loading Controls

Loading controls

Loading control primary antibodies target proteins which typically exhibit ubiquitous expression. There use is valuable in determining relative protein concentration and in determining the successful transfer of protein in western blots.

Rabbit Monoclonals

Rabbit monoclonals

Primary rabbit monoclonal antibodies can exhibit a greater binding affinity to antigens than those from rodents such as mice. Using rabbit monoclonal antibodies is particularly advantageous for more accurate immunohistochemistry results.

Phospho Antibodies

Phospho-antibodies

Protein phosphorylation plays a significant role in a wide range of post-translational cellular processes including cell growth and proliferation, metabolism, physiological regulation and cell signalling.

Knockout

KO-validated antibodies

        In a knockout validation, antibody specificity is confirmed by testing the antibody of interest against a sample from a tissue or cell line which does not express the protein of interest as well as a positive control which does.

Goat Primary Antibodies

Goat primary antibodies

Goat primary antibodies are ideal for large scale projects at a low cost. We have over 3000 goat primary antibodies suitable for various applications.

Tag antibodies

Tag antibodies

Tag antibodies are frequently used with recombinant protein cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. One of the most popular is our Anti-mCherry-Tag antibody used widely in gene expression assays.

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  • Anti-HA-tag antibody (KLH) [STJ90107]

    Western Blot (WB) analysis using HA-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing HA tag (1) and untransfected (2).
    Clonality: Polyclonal
    Host: Rabbit
    Applications: ELISA, WB
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  • Anti-TWIST1 Antibody [STJ29453]

    Western blot analysis of extracts of various cell lines, using TWIST1 antibody (STJ29453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in T
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: IF, WB
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  • Anti-RARA Antibody [STJ26160]

    Western blot analysis of extracts of various cell lines, using RARA antibody (STJ26160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBS
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: IF, IHC, WB
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  • Anti-RUNX1 Antibody [STJ25422]

    Western blot analysis of extracts of SH-SY5Y cells, using RUNX1 antibody (STJ25422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. D
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: IF, IHC, WB
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  • Anti-CASP1 Antibody [STJ22893]

    Western blot analysis of extracts of various cell lines, using CASP1 Antibody (STJ22893) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TB
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: WB
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  • Anti-CTTN Antibody [STJ70093]

    STJ70093 (0.1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Bovine, Canine, Human, Mouse, Rat
    Applications: ELISA, WB
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  • Anti-TMPO antibody [STJ98661]

    Western Blot (WB) analysis of Mouse Kidney, HepG2 cells using Antibody diluted at 1:800.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, WB
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  • Anti-Acetyl Lysine antibody [STJ97181]

    Western Blot (WB) analysis of 1) HeLa, 2) 3T3, 3) Rat Brain, diluted at 1:2000.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: All
    Applications: WB
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  • Anti-HSP70 antibody [STJ96944]

    Western blot (WB) analysis of skeletal muscle cells, using HSP70 monoclonal antibody diluted at 1:2000.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Human, Mouse, Rat
    Applications: IF, WB
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  • Anti-UCH-L1 antibody [STJ96177]

    Western Blot (WB) analysis of specific cells using UCH-L1 polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, WB
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  • Anti-PDHA1 antibody [STJ95006]

    Western Blot (WB) analysis of specific cells using PDHA1 polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, WB
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  • Anti-OTUB2 antibody [STJ94840]

    Western Blot (WB) analysis of specific cells using OTUB2 polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, WB
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