Loading Control

 

St John's Laboratory offers a variety of primary polyclonal and monoclonal antibodies. With numerous cited primary and secondary antibodies available from our catalogue of more than 30,000 antibodies we have you covered for your western blot, immunofluorescence, ELISA and other assay needs. So whether you are after rabbit monoclonals, goat polyclonals or ELISA kits, we provide coverage for the majority of research areas.

 

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Featured Products

Feature Product - Anti-Beta Actin Antibody (STJ96930)

Feature Product - Anti-Beta Actin primary antibody (STJ96930)

Mouse monoclonal Anti-Beta Actin primary antibody with 4 reviews over 4-star, covering multiple applications and multiple species, at high dilution.

IHC Guaranteed Antibodies

IHC-Guarantee primary antibodies

We have selected a wide range of IHC-validated primary antibodies suitable for immunohistochemistry applications, covering pathology, cancer research, immunology and other usual biomarkers.

  Loading Controls

Loading controls

Loading control primary antibodies target proteins which typically exhibit ubiquitous expression. There use is valuable in determining relative protein concentration and in determining the successful transfer of protein in western blots.

Rabbit Monoclonals

Rabbit monoclonals

Primary rabbit monoclonal antibodies can exhibit a greater binding affinity to antigens than those from rodents such as mice. Using rabbit monoclonal antibodies is particularly advantageous for more accurate immunohistochemistry results.

Phospho Antibodies

Phospho-antibodies

Protein phosphorylation plays a significant role in a wide range of post-translational cellular processes including cell growth and proliferation, metabolism, physiological regulation and cell signalling.

Knockout

KO-validated antibodies

        In a knockout validation, antibody specificity is confirmed by testing the antibody of interest against a sample from a tissue or cell line which does not express the protein of interest as well as a positive control which does.

Goat Primary Antibodies

Goat primary antibodies

Goat primary antibodies are ideal for large scale projects at a low cost. We have over 3000 goat primary antibodies suitable for various applications.

Tag antibodies

Tag antibodies

Tag antibodies are frequently used with recombinant protein cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. One of the most popular is our Anti-mCherry-Tag antibody used widely in gene expression assays.

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  • Anti-mCherry-Tag antibody [STJ34373]

    Immunofluorescence of A549 cells transfected with mCherry vector were stained with Anti-mcherry Antibody (green). Overlap of green and red (natural mCherry exposure) demonstrates mCherry-tag specificity.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: All
    Applications: FC, IF, IHC, IP, WB
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  • Anti-His-Tag antibody [STJ96906]

    Immunoprecipitation (IP) analysis: 5ug His Mouse IgG1 per ml Lysate, WB 1:3000.
    Clonality: Monoclonal
    Host: Mouse
    Applications: IF, IP, WB
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  • Anti-Flag-Tag antibody [STJ96903]

    Immunoprecipitation (IP) analysis: 5ug Flag Mouse IgG1 per ml Lysate, WB 1:5000.
    Clonality: Monoclonal
    Host: Mouse
    Applications: IF, IP, WB
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  • Anti-Flag-Tag antibody [STJ96927]

    Western Blot (WB) analysis of Recombinant protein, diluted at 1) 1:5000, 2) 1:10000.
    Clonality: Monoclonal
    Host: Mouse
    Applications: WB
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  • Anti-Strep-Tag antibody [STJ96918]

    Western Blot (WB) analysis: 1ug Strep II fusion protein and primary antibody dilution at 1) 1:5000, 2) 1:10000.
    Clonality: Monoclonal
    Host: Mouse
    Applications: WB
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  • Anti-Myc-Tag antibody [STJ96904]

    Immunoprecipitation (IP) analysis: 3ug Flag Mouse IgG1 per ml Lysate, WB 1:5000.
    Clonality: Monoclonal
    Host: Mouse
    Applications: IF, IP, WB
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  • Anti-His-tag antibody [STJ99289]

    Western blot detection of His-tag in various bacterial lysates, which containing His-tagged proteins (MW:30 kDa, 45 kDa, 72 kDa, 44 kDa and 40 kDa respectively) using anti-his antibody (1:1000 diluted).
    Clonality: Monoclonal
    Host: Mouse
    Applications: ELISA, WB
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  • Anti-Myc-Tag antibody [STJ96926]

    Western Blot (WB) analysis of Recombinant protein, diluted at at 1) 1:5000, 2) 1:10000.
    Clonality: Monoclonal
    Host: Mouse
    Applications: WB
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  • Anti-His-Tag antibody [STJ96925]

    Western Blot (WB) analysis of His recombinant protein, diluted at 1) 1:10000, 2) 1:20000.
    Clonality: Monoclonal
    Host: Mouse
    Applications: WB
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  • Anti-S-Tag antibody [STJ96919]

    Western Blot (WB) analysis: 2ug S-Tag fusion protein and primary antibody dilution at 1) 1:5000, 2) 1:10000.
    Clonality: Monoclonal
    Host: Mouse
    Applications: WB
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  • Anti-T7-Tag antibody [STJ96914]

    Western Blot (WB) analysis: 0.5ug T7 fusion protein and primary antibody dilution at 1) 1:3000, 2) 1:5000, 3) 1:10000.
    Clonality: Monoclonal
    Host: Mouse
    Applications: WB
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  • Anti-VSV-G-Tag antibody [STJ96908]

    Immunoprecipitation (IP) analysis using 5ug VSV-G mouse IgG1 per 1ml of lysate. Anti-VSV-G-Tag antibody was diluted at 1:5000.
    Clonality: Monoclonal
    Host: Mouse
    Applications: IF, IP, WB
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