Loading Control

 

St John's Laboratory offers a variety of primary polyclonal and monoclonal antibodies. With numerous cited primary and secondary antibodies available from our catalogue of more than 30,000 antibodies we have you covered for your western blot, immunofluorescence, ELISA and other assay needs. So whether you are after rabbit monoclonals, goat polyclonals or ELISA kits, we provide coverage for the majority of research areas.

 

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Featured Products

Feature Product - Anti-Beta Actin Antibody (STJ96930)

Feature Product - Anti-Beta Actin primary antibody (STJ96930)

Mouse monoclonal Anti-Beta Actin primary antibody with 4 reviews over 4-star, covering multiple applications and multiple species, at high dilution.

IHC Guaranteed Antibodies

IHC-Guarantee primary antibodies

We have selected a wide range of IHC-validated primary antibodies suitable for immunohistochemistry applications, covering pathology, cancer research, immunology and other usual biomarkers.

  Loading Controls

Loading controls

Loading control primary antibodies target proteins which typically exhibit ubiquitous expression. There use is valuable in determining relative protein concentration and in determining the successful transfer of protein in western blots.

Rabbit Monoclonals

Rabbit monoclonals

Primary rabbit monoclonal antibodies can exhibit a greater binding affinity to antigens than those from rodents such as mice. Using rabbit monoclonal antibodies is particularly advantageous for more accurate immunohistochemistry results.

Phospho Antibodies

Phospho-antibodies

Protein phosphorylation plays a significant role in a wide range of post-translational cellular processes including cell growth and proliferation, metabolism, physiological regulation and cell signalling.

Knockout

KO-validated antibodies

        In a knockout validation, antibody specificity is confirmed by testing the antibody of interest against a sample from a tissue or cell line which does not express the protein of interest as well as a positive control which does.

Goat Primary Antibodies

Goat primary antibodies

Goat primary antibodies are ideal for large scale projects at a low cost. We have over 3000 goat primary antibodies suitable for various applications.

Tag antibodies

Tag antibodies

Tag antibodies are frequently used with recombinant protein cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. One of the most popular is our Anti-mCherry-Tag antibody used widely in gene expression assays.

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  • Anti-HA-tag antibody (KLH) [STJ90107]

    Western Blot (WB) analysis using HA-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing HA tag (1) and untransfected (2).
    Clonality: Polyclonal
    Host: Rabbit
    Applications: ELISA, WB
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  • Anti-His-tag antibody (KLH) [STJ90106]

    Western Blot (WB) analysis using His-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing His tag (1) and untransfected (2).
    Clonality: Polyclonal
    Host: Rabbit
    Applications: ELISA, WB
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  • Anti-FLAG-tag antibody (KLH) [STJ90108]

    Western Blot (WB) analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2).
    Clonality: Polyclonal
    Host: Rabbit
    Applications: ELISA, WB
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  • Anti-Collagen 1, alpha 1 propeptide antibody (KLH) [STJ120037]

    Immunostaining of fibrotic mouse lung tissue showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized, using Anti-Collagen 1, alpha 1 propeptide (KLH) polyclonal antibody (STJ120037).
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Amphibians, Avian, Fish, Human, Rat
    Applications: IHC, WB
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  • Anti-Myc-tag antibody (KLH) [STJ90105]

    Western Blot (WB) analysis using Myc-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing Myc tag (1) and untransfected (2).
    Clonality: Polyclonal
    Host: Rabbit
    Applications: ELISA, WB
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  • Anti-Phospho-Kv3.1 (Ser503) antibody (KLH) [STJ120198]

    Immunostaining of medial nucleus of the trapezoid body (MNTB) cells with the phospho-Ser503 Kv31 subunit antibody The left panel shows control cells The right panel shows cells that have been exposed to the protein kinase C activator PMA, using Anti-Phosp
    Clonality: Polyclonal
    Host: Rabbit
    Applications: IHC, WB
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  • Anti-Phospho-MEK1/2 (Ser218/222) antibody (KLH) [STJ120127]

    Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45 kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222 The cells were either serum starved (-) or incubated in the presence of serum (+) Immunolabeling of an additional band
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Avian, Bovine, Canine, Rat, Simian, Xenopus, Zebrafish
    Applications: WB
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  • Anti-Dynamin antibody (KLH) [STJ120059]

    Western Blot of rat cortical lysate showing labeling of the ~95 kDa dynamin protein, using Anti-Dynamin (KLH) polyclonal antibody (STJ120059).
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Avian, Bovine, Human, Mouse, Simian
    Applications: WB
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  • Anti-NMDAR1 C2 antibody (KLH) [STJ120153]

    Western blot of 10 µg of HEK 293 cells specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor containing the C2’ splice variant insert (lanes 3 and 4) 1) No NR1 expression. 2) NR1 subunit containing only the C2 Insert. 3) NR1 subunit con
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Bovine, Human
    Applications: IHC, WB
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  • Anti-Phospho-Olig2 (Ser10/13/14) antibody (KLH) [STJ120179]

    Western blot of rat neonatal brain lysate showing specific immunolabeling of the ~32 kDa form of the Olig2 protein phosphorylated at Ser10,13,14 in the first lane (-) Phosphospecificity was shown in the second lane (+) where Immunolabeling was blocked by
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Guinea Pig, Human, Simian, Zebrafish
    Applications: WB
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  • Anti-Phospho-REDD1 (Thr23/25) antibody (KLH) [STJ120212]

    Western blot of jurkat cell lysate showing specific immunolabeling of the ~34 kDa REDD1 protein phosphorylated at Thr23/25 in the first lane (-) Phosphospecificity was shown in the second lane (+) where Immunolabeling was blocked by the phosphopeptide use
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Simian
    Applications: WB
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  • Anti-Phospho-p62 (Thr269/Ser272) antibody (KLH) [STJ120183]

    Western blot of Jurkat cell lysate showing specific immunolabeling of the ~62 kDa p62 phosphorylated at Thr269/Ser272 in the first lane (-) Phosphospecificity was shown in the second lane (+) where immunolabeling was blocked by preadsorption of the phosph
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Simian
    Applications: WB
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