Loading Control

 

St John's Laboratory offers a variety of primary polyclonal and monoclonal antibodies. With numerous cited primary and secondary antibodies available from our catalogue of more than 30,000 antibodies we have you covered for your western blot, immunofluorescence, ELISA and other assay needs. So whether you are after rabbit monoclonals, goat polyclonals or ELISA kits, we provide coverage for the majority of research areas.

 

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Featured Products

Feature Product - Anti-Beta Actin Antibody (STJ96930)

Feature Product - Anti-Beta Actin primary antibody (STJ96930)

Mouse monoclonal Anti-Beta Actin primary antibody with 4 reviews over 4-star, covering multiple applications and multiple species, at high dilution.

IHC Guaranteed Antibodies

IHC-Guarantee primary antibodies

We have selected a wide range of IHC-validated primary antibodies suitable for immunohistochemistry applications, covering pathology, cancer research, immunology and other usual biomarkers.

  Loading Controls

Loading controls

Loading control primary antibodies target proteins which typically exhibit ubiquitous expression. There use is valuable in determining relative protein concentration and in determining the successful transfer of protein in western blots.

Rabbit Monoclonals

Rabbit monoclonals

Primary rabbit monoclonal antibodies can exhibit a greater binding affinity to antigens than those from rodents such as mice. Using rabbit monoclonal antibodies is particularly advantageous for more accurate immunohistochemistry results.

Phospho Antibodies

Phospho-antibodies

Protein phosphorylation plays a significant role in a wide range of post-translational cellular processes including cell growth and proliferation, metabolism, physiological regulation and cell signalling.

Knockout

KO-validated antibodies

        In a knockout validation, antibody specificity is confirmed by testing the antibody of interest against a sample from a tissue or cell line which does not express the protein of interest as well as a positive control which does.

Goat Primary Antibodies

Goat primary antibodies

Goat primary antibodies are ideal for large scale projects at a low cost. We have over 3000 goat primary antibodies suitable for various applications.

Tag antibodies

Tag antibodies

Tag antibodies are frequently used with recombinant protein cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. One of the most popular is our Anti-mCherry-Tag antibody used widely in gene expression assays.

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  • Anti-beta-Actin antibody [STJ99028]

    Western blot detection of beta actin in C6, 3T3, COS7, CHO-k1, K562 and Goat muscle cell lysates using beta actin mouse mAb (1:10000 diluted). Predicted band size: 45KDa. Observed band size: 45KDa.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Arabidopsis, Chlamydomonas, Cucumber, Drosophila, Goat, Hamster, Human, Mouse, Pichia pastoris, Rabbit, Rat, Rice Leaf, Saccharomyces cerevisiae, Simian, Zebrafish
    Applications: ELISA, WB
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  • Anti-HSP90B1 Antibody [STJ70946]

    STJ70946 (3.8µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Bovine, Canine, Human, Mouse, Rat, Swine, Zebrafish
    Applications: ELISA, IHC, WB
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  • Anti-Actinin-alpha antibody [STJ98460]

    Western Blot (WB) analysis using Actinin-alpha2 Monoclonal Antibody against HepG2 cell lysate.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Avian, Bovine, Canine, Human, Rat, Swine, Zebrafish
    Applications: IF, WB
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  • Anti-beta-Actin antibody [STJ130015]

    Immunohistochemical analysis of paraffin-embedded human colon tissue with rabbit monoclonal anti-beta actin antibody [4C4] at 1:1000 dilution, using Anti-beta-Actin monoclonal antibody (STJ130015).
    Clonality: Monoclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat, Swine, Zebrafish
    Applications: ELISA, IF, WB
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  • Anti-CDH11 antibody [STJ140122]

    Western Blot was carried out on 3 day zebrafish embryo cells, using endogenous Anti-CDH11 polyclonal antibody at a 1:2,000 dilution, with lysates of 50 µg per lane and secondary antibody rabbit anti-goat IgG (HRP) at 1:10,000 dilution.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Zebrafish
    Applications: WB
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  • Anti-CDH11 antibody [STJ140121]

    Western Blot was carried out on 3 day zebrafish embryo cells, using Anti-CDH11 polyclonal antibody at a 1:2,000 dilution, with lysates of 50 µg per lane and secondary antibody rabbit anti-goat IgG (HRP) at 1:10,000 dilution.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Zebrafish
    Applications: WB
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  • Anti-Phospho-MEK1/2 (Ser218/222) antibody (KLH) [STJ120127]

    Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45 kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222 The cells were either serum starved (-) or incubated in the presence of serum (+) Immunolabeling of an additional band
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Avian, Bovine, Canine, Rat, Simian, Xenopus, Zebrafish
    Applications: WB
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  • Anti-Phospho-Olig2 (Ser10/13/14) antibody (KLH) [STJ120179]

    Western blot of rat neonatal brain lysate showing specific immunolabeling of the ~32 kDa form of the Olig2 protein phosphorylated at Ser10,13,14 in the first lane (-) Phosphospecificity was shown in the second lane (+) where Immunolabeling was blocked by
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Guinea Pig, Human, Simian, Zebrafish
    Applications: WB
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  • Anti-Phospho-Cardiac Troponin I (Ser43) antibody (KLH) [STJ120257]

    Western blot of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein phosphorylated at Ser43 in the first lane (-) Phosphospecificity was shown in the second lane (+) where the immunolabeling was greatly decreased b
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Bovine, Canine, Human, Sheep, Simian, Xenopus, Zebrafish
    Applications: WB
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  • Anti-Phospho-SNAP25 (Ser187) antibody (KLH) [STJ120225]

    Western blot of rat hippocampal lysate showing specific immunolabeling of the ~25 kDa SNAP25 phosphorylated at Ser187 in the first lane (-) Phosphospecificity was shown in the second lane (+) where the immunolabeling was completely eliminated by lysate tr
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Guinea Pig, Human, Simian, Zebrafish
    Applications: WB
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  • Anti-Phospho-Polo-Like Kinase Kinase (Ser482/486/490) antibody (KLH) [STJ120196]

    Western blot of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-) Phosphospecificity was shown in the second lane (+) where the immunolabeling was
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat, Zebrafish
    Applications: WB
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  • Anti-Phospho-Tryptophan Hydroxylase (Ser260) antibody (KLH) [STJ120261]

    Western blot of rat brainstem lysate showing specific immunolabeling of the ~55 kDa TPH protein phosphorylated at Ser260 in lane one (-) Phosphospecificity was shown in the second lane (P) where immunolabeling was blocked by preadsorption with the phospho
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Avian, Bovine, Canine, Mouse, Zebrafish
    Applications: WB
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