Loading Control

 

St John's Laboratory offers a variety of primary polyclonal and monoclonal antibodies. With numerous cited primary and secondary antibodies available from our catalogue of more than 30,000 antibodies we have you covered for your western blot, immunofluorescence, ELISA and other assay needs. So whether you are after rabbit monoclonals, goat polyclonals or ELISA kits, we provide coverage for the majority of research areas.

 

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Featured Products

Feature Product - Anti-Beta Actin Antibody (STJ96930)

Feature Product - Anti-Beta Actin primary antibody (STJ96930)

Mouse monoclonal Anti-Beta Actin primary antibody with 4 reviews over 4-star, covering multiple applications and multiple species, at high dilution.

IHC Guaranteed Antibodies

IHC-Guarantee primary antibodies

We have selected a wide range of IHC-validated primary antibodies suitable for immunohistochemistry applications, covering pathology, cancer research, immunology and other usual biomarkers.

  Loading Controls

Loading controls

Loading control primary antibodies target proteins which typically exhibit ubiquitous expression. There use is valuable in determining relative protein concentration and in determining the successful transfer of protein in western blots.

Rabbit Monoclonals

Rabbit monoclonals

Primary rabbit monoclonal antibodies can exhibit a greater binding affinity to antigens than those from rodents such as mice. Using rabbit monoclonal antibodies is particularly advantageous for more accurate immunohistochemistry results.

Phospho Antibodies

Phospho-antibodies

Protein phosphorylation plays a significant role in a wide range of post-translational cellular processes including cell growth and proliferation, metabolism, physiological regulation and cell signalling.

Knockout

KO-validated antibodies

        In a knockout validation, antibody specificity is confirmed by testing the antibody of interest against a sample from a tissue or cell line which does not express the protein of interest as well as a positive control which does.

Goat Primary Antibodies

Goat primary antibodies

Goat primary antibodies are ideal for large scale projects at a low cost. We have over 3000 goat primary antibodies suitable for various applications.

Tag antibodies

Tag antibodies

Tag antibodies are frequently used with recombinant protein cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. One of the most popular is our Anti-mCherry-Tag antibody used widely in gene expression assays.

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  • Anti-GAPDH antibody [STJ96931]

    Western blot (WB) analysis of Hela, rat brain, rabbit muscle, sheep muscle and mouse brain cells, using monoclonal Anti-GAPDH Antibody (STJ96931) at 1:10000.
    Clonality: Monoclonal
    Host: Mouse
    Reactivity: Bovine, Canine, Hamster, Human, Insect, Mouse, Rabbit, Rat, Sheep, Simian, Swine, Yeast
    Applications: IF, IHC, WB
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    Rating:
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  • Anti-ZO1 antibody [STJ140055]

    Immunofluorescence was carried out on primary RPE cells, using Anti-ZO1 polyclonal antibody at a 1:100 dilution.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Avian, Bovine, Canine, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Simian, Swine
    Applications: IF, IHC, WB
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  • Anti-PRDM1 Antibody [STJ72074]

    STJ72074 (3.8µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Bovine, Canine, Human, Mouse, Rat, Swine
    Applications: ELISA, WB
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  • Anti-creatine kinase M-type Antibody [STJ72970]

    STJ72970 (0.03µg/ml) staining of Human (A) and Mouse (B) Heart lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Bovine, Canine, Human, Mouse, Rat, Swine
    Applications: ELISA, WB
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  • Anti-CHRM2 Antibody [STJ72844]

    STJ72844 (2µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Bovine, Canine, Human, Mouse, Swine
    Applications: ELISA, WB
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  • Anti-Hdac1 Antibody [STJ72765]

    STJ72765 (0.3µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Bovine, Human, Mouse, Rat, Swine
    Applications: ChIP, ELISA, WB
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  • Anti-NPPA Antibody [STJ72680]

    STJ72680 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Bovine, Canine, Human, Mouse, Rat, Swine
    Applications: ELISA, IHC, WB
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  • Anti-NGFR Antibody [STJ72666]

    STJ72666 (1µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Bovine, Human, Rat, Swine
    Applications: ELISA, WB
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  • Anti-JAG1 Antibody [STJ72591]

    STJ72591 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Bovine, Canine, Human, Mouse, Rat, Swine
    Applications: ELISA, IF, IHC
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  • Anti-SOX10 Antibody [STJ71694]

    STJ71694 (0.1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Bovine, Canine, Human, Mouse, Rat, Swine
    Applications: ELISA, WB
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  • Anti-NLRP3 Antibody [STJ70965]

    STJ70965 (2µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Bovine, Canine, Human, Mouse, Rat, Swine
    Applications: ELISA, IHC
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  • Anti-HSP90B1 Antibody [STJ70946]

    STJ70946 (3.8µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Bovine, Canine, Human, Mouse, Rat, Swine, Zebrafish
    Applications: ELISA, IHC, WB
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