Anti-beta-Actin antibody

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Product name Anti-beta-Actin antibody
Short Description Rabbit monoclonal against beta-Actin
Description Beta (beta) actin is a member of highly conserved actins that are involved in cell motility, structure, and integrity. Beta actin exists in two forms in the cytoplasm: globular actin monomer (G-actin) with a molecular weight of approximately 42 kDa, and the fibrous polymer (F-actin). Due to its ubiquitous expression in eukaryotic cells, beta actin is widely used as a loading control for western blotting, which guides the user to identify the cell integrity. Defects in beta actin result in dystonia juvenile-onset (DYTJ), which often leads to abnormal posture, dopa-unresponsive dystonia, developmental malformations and loss of hearing. Studies have also demonstrated the association of actin with malignancies such as breast cancer and lymphoma.
Applications ELISA, IF, WB
Specificity human, mouse, rat, pig and zebrafish beta actin
Protein Name Actin Beta
Immunogen Full length recombinant human beta actin protein
Storage Instruction Store Anti-beta-Actin Antibody at -20°C, and avoid repeated freeze-thaw cycles.
Note For Research Use Only (RUO)
Validated Application WB ELISA IF
Host Rabbit
Clonality Monoclonal
Clone ID 4C4
Reactivity Human, Mouse, Rat, Swine, Zebrafish
Conjugation Unconjugated
Purification Protein A agarose affinity chromatography of tissue culture supernatant
Isotype IgG
Formulation PBS with 0.09% sodium azide, and 50% glycerol at pH7.4
Gene ID 60
Gene Symbol ACTB
Molecular Weight 42 kDa
Database Links HGNC:132
OMIM:102630
Alternative Names Actin Beta
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Post-translational Modifications PTM: ISGylated. {ECO:0000269|PubMed:16139798}.; PTM: Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization . {ECO:0000250}.; PTM: Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. {ECO:0000269|PubMed:23673617}.; PTM: (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). {ECO:0000305|PubMed:19015515, ECO:0000305|PubMed:26228148}.
Cellular Localization Cytoplasm, cytoskeleton {ECO:0000269|PubMed:17289661}. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Swiss-Prot Key P60709_HUMAN
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