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Anti-Cleaved-Caspase-3 p17 (D175) antibody

[STJ90005] Download PDF Print Data Sheet

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Product name Anti-Cleaved-Caspase-3 p17 (D175) antibody
Short Description Rabbit polyclonal to Cleaved-Caspase-3 p17 (D175).
Description Caspase-3 p17 is a protein encoded by the CASP3 gene which is approximately 31,6 kDa. Caspase-3 p17 is localised to the cytoplasm. It is involved in the TNFR1 pathway, apoptosis modulation and signalling and respiratory electron transport. This protein falls under the cysteine-aspartic acid protease family. It is a sequential activatior of caspases and plays a central role in the execution-phase of cell apoptosis. Caspase-3 p17 is highly expressed in lung, spleen, heart, liver and kidney. Mutations in the CASP3 gene may result in bladder urothelial carcinoma and primary effusion lymphoma. STJ90005 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of fragment activated Caspase-3 p17 protein resulting from cleavage adjacent to D175.
Applications ELISA, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Specificity Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-3 p17 protein resulting from cleavage adjacent to D175.
Protein Name Caspase-3
Cysteine protease CPP32
Protein Yama
SREBP cleavage activity 1
Caspase-3 subunit p17
Caspase-3 subunit p12
Immunogen Synthesized peptide derived from human Caspase-3 p17
Immunogen Region 100-180 aa, Internal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 836
Gene Symbol CASP3
Molecular Weight 35/17 kDa
Database Links HGNC:1504
Alternative Names Cleaved-Caspase-3 p17 (D175)
Caspase-3 antibody
CASP-3 antibody
Apopain antibody
Cysteine protease CPP32 antibody
CPP-32 antibody
Protein Yama antibody
SREBP cleavage activity 1 antibody
SCA-1 antibody
Caspase-3 subunit p17 antibody
Caspase-3 subunit p12 antibody
CPP32 antibody
Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Post-translational Modifications Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.; S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
Cellular Localization Cytoplasm.
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Swiss-Prot Key P42574_HUMAN
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