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Anti-Cleaved-Notch 1 (V1754) antibody

[STJ90066] Download PDF Print Data Sheet
5-7 days

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Product name Anti-Cleaved-Notch 1 (V1754) antibody
Short Description Rabbit polyclonal to Cleaved-Notch 1 (V1754).
Description Notch 1 is a protein encoded by the NOTCH1 gene which is approximately 272,5 kDa. Notch 1 is localised to the cell membrane. It is involved in the notch signalling pathway and Notch 2 activation and transmission of signal to the nucleus. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Notch 1 is expressed in most adult tissues but is found mainly in lymphoid tissues. Mutations in the NOTCH1 gene may result in aortic valve disease and Adams-Oliver syndrome. STJ90066 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of fragment of activated Notch 1 protein resulting from cleavage adjacent to V1754.
Applications ELISA, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Specificity Cleaved-Notch 1 (V1754) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 1 protein resulting from cleavage adjacent to V1754.
Protein Name Neurogenic locus notch homolog protein 1
Notch 1
hN1
Translocation-associated notch protein TAN-1
Notch 1 extracellular truncation
NEXT
Notch 1 intracellular domain
NICD
Immunogen Synthesized peptide derived from human Notch 1
Immunogen Region 1710-1790 aa, Internal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Mouse, Rat, Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 4851
Gene Symbol NOTCH1
Molecular Weight 110 kDa
Database Links HGNC:7881
OMIM:109730
Alternative Names Cleaved-Notch 1 (V1754)
Neurogenic locus notch homolog protein 1 antibody
Notch 1 antibody
hN1 antibody
Translocation-associated notch protein TAN-1 antibody
Notch 1 extracellular truncation antibody
NEXT antibody
Notch 1 intracellular domain antibody
NICD antibody
TAN1 antibody
Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
Post-translational Modifications Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by ADAM17 to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). Following endocytosis, this fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. O-glycosylated on the EGF-like domains . Contains both O-linked fucose and O-linked glucose in the EGF-like domains 11, 12 and 13, which are interacting with the residues on DLL4 . O-linked glycosylation by GALNT11 is involved in determination of left/right symmetry: glycosylation promotes activation of NOTCH1, possibly by promoting cleavage by ADAM17, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO) . Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH. Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch. Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN . Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD .
Cellular Localization Cell membrane Notch 1 intracellular domain: Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
Tissue Specificity In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
Swiss-Prot Key P46531_HUMAN
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