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Anti-Egr-2 antibody

[STJ92863] Download PDF Print Data Sheet
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Product name Anti-Egr-2 antibody
Short Description Rabbit polyclonal to Egr-2.
Description Egr-2 is a protein encoded by the EGR2 gene which is approximately 50,3 kDa. Egr-2 is localised to the nucleus and is involved in toll-like receptor signalling pathway, HTLV-I infection, viral carcinogenesis and developmental biology. It is a sequence-specific DNA-binding transcription factor, it binds to two specific DNA sites located in the promoter region of HOXA4. It is also an E3 SUMO-protein ligase, helping SUMO1 conjugate to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 own transcriptional activity. Egr-2 is expressed in the cells of the nervous system. Mutations in the EGR2 gene may result in congenital hypomyelinatingand Charcot-Marie-tooth disease. STJ92863 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. This antibody binds to endogenous Egr-2.
Applications ELISA, IF, IHC-p, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Specificity Egr-2 Polyclonal Antibody detects endogenous levels of Egr-2 protein.
Protein Name Early growth response protein 1
EGR-1
AT225
Nerve growth factor-induced protein A
NGFI-A
Transcription factor ETR103
Transcription factor Zif268
Zinc finger protein 225
Zinc finger protein Krox-24
Immunogen Synthesized peptide derived from human Egr-2
Immunogen Region 340-420 aa, C-terminal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Mouse, Rat, Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1958
Gene Symbol EGR1
Molecular Weight 50 kDa
Database Links HGNC:3238
OMIM:128990
Alternative Names Early growth response protein 1 antibody
EGR-1 antibody
AT225 antibody
Nerve growth factor-induced protein A antibody
NGFI-A antibody
Transcription factor ETR103 antibody
Transcription factor Zif268 antibody
Zinc finger protein 225 antibody
Zinc finger protein Krox-24 antibody
KROX24 antibody
ZNF225 antibody
Function Transcriptional regulator . Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes . Binds double-stranded target DNA, irrespective of the cytosine methylation status . Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary .
Cellular Localization Nucleus Cytoplasm
Tissue Specificity Detected in neutrophils (at protein level).
Sequence and Domain Family Binds to DNA motifs with the sequence 5'-GCG(T/G)GGGCG-3' via its C2H2-type zinc fingers . The first, most N-terminal zinc finger binds to the 3'-GCG motif, the middle zinc finger interacts with the central TGG motif, and the C-terminal zinc finger binds to the 5'-GCG motif. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Has reduced affinity for target DNA where the cytosines have been oxidized to 5-hydroxymethylcytosine. Does not bind target DNA where the cytosines have been oxidized to 5-formylcytosine or 5-carboxylcytosine .
Swiss-Prot Key P18146_HUMAN
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