Anti-EIF2AK2 Antibody

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Product name Anti-EIF2AK2 Antibody
Short Description Rabbit polyclonal against EIF2AK2
Description The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.
Applications IF, IHC, WB
Dilution range WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Protein Name Interferon-induced
double-stranded RNA-activated protein kinase
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human Eif2ak2 (NP_002750.1).
Storage Instruction Store at -20C. Avoid freeze / thaw cycles.
Note For Research Use Only (RUO).
Validated Application WB IHC IF
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Gene ID 5610
Gene Symbol EIF2AK2
Molecular Weight 62.094 kDa
Database Links HGNC:9437
OMIM:176871
Reactome:R-HSA-1169408
Alternative Names Interferon-induced
double-stranded RNA-activated protein kinase
Function IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation, Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1), Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis, Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11, In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation, Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs, Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6, Can act as both a positive and negative regulator of the insulin signaling pathway (ISP), Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2), Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes, Can trigger apoptosis via FADD-mediated activation of CASP8, Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin,
Post-translational Modifications Autophosphorylated on several Ser, Thr and Tyr residues, Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization, Autophosphorylation apparently leads to the activation of the kinase, Tyrosine autophosphorylation is essential for efficient dsRNA-binding, dimerization, and kinase activation,
Cellular Localization Cytoplasm, Nucleus, Cytoplasm, perinuclear region,
Tissue Specificity Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues, Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain, Elevated levels seen in breast and colon carcinomas,and which correlates with tumor progression and invasiveness or risk of progression
Swiss-Prot Key P19525
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