Anti-Factor VIII antibody

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Product name Anti-Factor VIII antibody
Description Rabbit polyclonal to Factor VIII.
Applications ELISA, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Specificity Factor VIII Polyclonal Antibody detects endogenous levels of Factor VIII protein.
Protein Name Coagulation factor VIII
Antihemophilic factor
Procoagulant component
Factor VIIIa heavy chain, 200 kDa isoform
Factor VIIIa heavy chain, 92 kDa isoform
Factor VIII B chain
Factor VIIIa light chain
Immunogen Synthesized peptide derived from human Factor VIII
Immunogen Region 2130-2210 aa, C-terminal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 2157
Gene Symbol F8
Molecular Weight 300 kDa
Database Links HGNC:3546
Alternative Names Coagulation factor VIII antibody
Antihemophilic factor antibody
AHF antibody
Procoagulant component antibodya heavy chain, 200 kDa isoform antibodya heavy chain, 92 kDa isoform antibody B chain antibodya light chain antibodyC antibody
Function Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.
Post-translational Modifications Sulfation on Tyr-1699 is essential for binding vWF.
Cellular Localization Secreted, extracellular space.
Sequence and Domain Family Domain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
Swiss-Prot Key P00451_HUMAN
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