Anti-FAS-L antibody

[STJ93042] Download PDF Print Data Sheet
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Product name Anti-FAS-L antibody
Short Description Rabbit polyclonal to FAS-L.
Description FAS-L is a protein encoded by the FASLG gene which is approximately 31,4 kDa. FAS-L is localised to the cell membrane and is involved in PEDF induced signalling, dimerization of procaspase-8, apoptosis modulation and signalling and the TNFR1 pathway. FAS-L is a member of the tumor necrosis factor superfamily. It is a cytokine that binds to TNFRSF6/FAS which is a receptor that transduces the apoptotic signal into cells. It may be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. FAS-L is expressed in the blood, spleen, lymph nodes, intestine and kidney. Mutations in the FASLG gene result in autoimmune lymphoproliferative syndrome 1B. STJ93042 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of FAS-L protein.
Applications ELISA, IF, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Specificity FAS-L Polyclonal Antibody detects endogenous levels of FAS-L protein.
Protein Name Tumor necrosis factor ligand superfamily member 6
Apoptosis antigen ligand
APTL
CD95 ligand
CD95-L
Fas antigen ligand
Fas ligand
FasL
CD antigen CD178
Tumor necrosis factor ligand superfamily member 6, membrane form
Tumor necrosis factor ligand superfamily member 6, soluble form
Receptor-binding FasL ectodomain
Soluble Fas ligand
sFasL
ADAM10-processed FasL form
APL
FasL intracellular domain
FasL ICD
Immunogen Synthesized peptide derived from human FAS-L
Immunogen Region 70-150 aa, Internal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IF IHC WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 356
Gene Symbol FASLG
Molecular Weight 33 kDa
Database Links HGNC:11936
OMIM:134638
Alternative Names Tumor necrosis factor ligand superfamily member 6 antibody
Apoptosis antigen ligand antibody
APTL antibody
CD95 ligand antibody
CD95-L antibody
Fas antigen ligand antibody
Fas ligand antibody
FasL antibody
CD antigen CD178 antibody
Tumor necrosis factor ligand superfamily member 6, membrane form antibody
Tumor necrosis factor ligand superfamily member 6, soluble form antibody
Receptor-binding FasL ectodomain antibody
Soluble Fas ligand antibody
sFasL antibody
ADAM10-processed FasL form antibody
APL antibody
FasL intracellular domain antibody
FasL ICD antibody
SPPL2A-processed FasL form antibody
SPA antibody
APT1LG1 antibody
CD95L antibody
FASL antibody
TNFSF6 antibody
Function Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells . Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development . Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses . TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance . Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis . Tumor necrosis factor ligand superfamily member 6, soluble form: Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways . Can induce apoptosis but does not appear to be essential for this process . FasL intracellular domain: Cytoplasmic form induces gene transcription inhibition.
Post-translational Modifications The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells. N-glycosylated . Glycosylation enhances apoptotic activity . Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization. Monoubiquitinated.
Cellular Localization Cell membrane Cytoplasmic vesicle lumen Lysosome lumen. Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination . Colocalizes with the SPPL2A protease at the cell membrane . Tumor necrosis factor ligand superfamily member 6, soluble form: Secreted. May be released into the extracellular fluid by cleavage from the cell surface. FasL intracellular domain: Nucleus. The FasL ICD cytoplasmic form is translocated into the nucleus.
Swiss-Prot Key P48023_HUMAN
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