Anti-FTO antibody (KLH)

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Product name Anti-FTO antibody (KLH)
Short Description KLH conjugated Mouse monoclonal to Alpha-ketoglutarate-dependent dioxygenase FTO
Description The FTO gene was the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which was involved in the stimulation of food intake (Fredricksson R et al., 2008). Deletion analysis of FTO gene in mice showed that Fto was functionally involved in the control of both energy intake and energy expenditure (Fischer J et al., 2009).
Applications IF, WB
Dilution range WB 1:1000; IF 1:100
Protein Name Alpha-ketoglutarate-dependent dioxygenase FTO
Fat mass and obesity-associated protein
Immunogen Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.
Immunogen Region N-term
Storage Instruction Store at -20°C, and avoid repeated freeze-thaw cycles.
Note For research use only.
Validated Application WB IF
Host Mouse
Clonality Monoclonal
Clone ID 5-2H10
Reactivity Bovine, Canine, Horse, Sheep, Simian
Conjugation KLH
Purification Anti-FTO antibody (KLH) was prepared using Protein G purification
Isotype IgG
Formulation 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Gene ID 79068
Gene Symbol FTO
Molecular Weight 58 kDa
Database Links HGNC:24678
OMIM:601665
Alternative Names Alpha-ketoglutarate-dependent dioxygenase FTO
Fat mass and obesity-associated protein
Function Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine (PubMed:18775698, PubMed:20376003). Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:26458103). Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation (PubMed:18775698, PubMed:20376003). In particular, it was involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed:26287746).
Cellular Localization Nucleus. Nucleus speckle.
Tissue Specificity Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
Sequence and Domain Family Belongs to the fto family.The 3D-structure of the Fe2OG dioxygenase domain was similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity was very low. As a consequence, the domain was not detected by protein signature databases.
Swiss-Prot Key Q9C0B1_HUMAN
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