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Anti-MARK1/2/3/4 antibody

[STJ94015] Download PDF Print Data Sheet

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Product name Anti-MARK1/2/3/4 antibody
Short Description Rabbit polyclonal to MARK1/2/3/4.
Description MARK1/2/3/4 is a protein encoded by the MARK1 gene which is approximately 89 kDa. MARK1/2/3/4 is localised to the cell membrane and cytoplasm. It is involved in cytoplasm signalling and glucose/energy metabolism. It plays a role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. MARK1/2/3/4 is highly expressed in the heart, skeletal muscle, brain and foetal kidney. Mutations in the MARK1 gene may result in Alzheimer disease. STJ94015 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of MARK1/2/3/4 protein.
Applications ELISA, IF, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Specificity MARK1/2/3/4 Polyclonal Antibody detects endogenous levels of MARK1/2/3/4 protein.
Protein Name Serine/threonine-protein kinase MARK1
MAP/microtubule affinity-regulating kinase 1
PAR1 homolog c
Par-1c
Par1c
Immunogen Synthesized peptide derived from human MARK1/2/3/4 around the non-phosphorylation site of T215.
Immunogen Region 150-230 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Monkey, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 4139
Gene Symbol MARK1
Molecular Weight 89 kDa
Database Links HGNC:6896
OMIM:606511
Alternative Names MARK1/2/3/4
Serine/threonine-protein kinase MARK1 antibody
MAP/microtubule affinity-regulating kinase 1 antibody
PAR1 homolog c antibody
Par-1c antibody
Par1c antibody
KIAA1477 antibody
MARK antibody
Function Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2, MAP4 and MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).
Post-translational Modifications Phosphorylation at Thr-613 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity . Phosphorylated at Thr-215 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-215 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-219 by GSK3-beta (GSK3B) inhibits the kinase activity.
Cellular Localization Cell membrane Cytoplasm, cytoskeleton. Appears to localize to an intracellular network.
Tissue Specificity Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.
Sequence and Domain Family The UBA domain does not seem to bind ubiquitin and ubiquitin-like and might play a role in regulating the enzyme conformation and localization. Activation of the kinase activity following phosphorylation at Thr-208 is accompanied by a conformational change that alters the orientation of the UBA domain with respect to the catalytic domain . The KA1 domain mediates binding to phospholipids and targeting to membranes. Binds phosphatidic acid (PA), phosphatidylserine (PtdSer) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).
Swiss-Prot Key Q9P0L2_HUMAN
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