Anti-MYOD1 antibody

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Product name Anti-MYOD1 antibody
Short Description Rabbit monoclonal against MYOD1
Description The MyoD1 gene belongs to a family of myogenic determination genes, including myogenin, myf-5 and MRF4, all of which encode transcription factors. Transfection of MyoD1 cDNA into non-muscle cells has been shown to activate expression of muscle specific genes and in some cases induce myogenesis. The MyoD1 protein is a 45 kD nuclear phosphoprotein which induces myogenesis through transcriptional activation of musclespecific genes. Nuclear expression of MyoD1 is restricted to skeletal muscle tissue and has been demonstrated to be a sensitive marker of myogenic differentiation. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymomal and a subset of Wilm’s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing’s sarcomas and alveolar soft part sarcomas.
Applications IHC
Dilution range 1:50-100
Protein Name Myoblast determination protein 1
Class C basic helix-loop-helix protein 1
bHLHc1
Myogenic factor 3
Myf-3
Immunogen A synthetic peptide corresponding to residues of human MyoD1 protein
Storage Instruction Store at 2-8°C for up to 1 year
Note For Research Use Only (RUO).
Validated Application IHC
Host Rabbit
Clonality Monoclonal
Reactivity Human
Conjugation Unconjugated
Isotype IgG
Formulation Antibody purified from ascites fluid by protein G chromatography in 0.2% BSA and 15mM sodium
Gene ID 4654
Gene Symbol MYOD1
Molecular Weight 35 kDa
Database Links HGNC:7611
OMIM:159970
Reactome:R-HSA-375170
Alternative Names Myoblast determination protein 1
Class C basic helix-loop-helix protein 1
bHLHc1
Myogenic factor 3
Myf-3
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis, Induces fibroblasts to differentiate into myoblasts, Interacts with and is inhibited by the twist protein, This interaction probably involves the basic domains of both proteins (By similarity),
Post-translational Modifications Phosphorylated by CDK9, This phosphorylation promotes its function in muscle differentiation.
Cellular Localization Nucleus
Swiss-Prot Key P15172
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