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Anti-p53 antibody

[STJ94890] Download PDF Print Data Sheet
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Product name Anti-p53 antibody
Short Description Rabbit polyclonal to p53.
Description p53 is a protein encoded by the TP53 gene which is approximately 43,6 kDa. p53 is localised to the cytoplasm, nucleus and endoplasmic reticulum. It is involved in RET signalling, CDK-mediated phosphorylation and removal of Cdc6, glioma and apoptotic pathways. It is a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. It responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest and changes in metabolism. p53 is expressed ubiquitously. Mutations in the TP53 gene may result in Li-Fraumeni syndrome and choroid plexus papilloma. STJ94890 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of p53 protein.
Applications ELISA, WB
Dilution range WB 1:500-1:2000
ELISA 1:20000
Specificity p53 Polyclonal Antibody detects endogenous levels of p53 protein.
Protein Name Cellular tumor antigen p53
Antigen NY-CO-13
Phosphoprotein p53
Tumor suppressor p53
Immunogen Synthesized peptide derived from human p53 around the non-acetylation site of K381
Immunogen Region 320-400 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Monkey, Mouse, Rat, Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 7157
Gene Symbol TP53
Molecular Weight 53 kDa
Database Links HGNC:11998
OMIM:133239
Alternative Names p53
Cellular tumor antigen p53 antibody
Antigen NY-CO-13 antibody
Phosphoprotein p53 antibody
Tumor suppressor p53 antibody
P53 antibody
Function Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 .
Post-translational Modifications Acetylated. Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylation by SIRT2 impairs its ability to induce transcription activation in a AKT-dependent manner. Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylated by HIPK1 . Phosphorylation at Ser-9 by HIPK4 increases repression activity on BIRC5 promoter. Phosphorylated on Thr-18 by VRK1. Phosphorylated on Ser-20 by CHEK2 in response to DNA damage, which prevents ubiquitination by MDM2. Phosphorylated on Ser-20 by PLK3 in response to reactive oxygen species (ROS), promoting p53/TP53-mediated apoptosis. Phosphorylated on Thr-55 by TAF1, which promotes MDM2-mediated degradation. Phosphorylated on Ser-33 by CDK7 in a CAK complex in response to DNA damage. Phosphorylated on Ser-46 by HIPK2 upon UV irradiation. Phosphorylation on Ser-46 is required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated on Ser-15 upon ultraviolet irradiation; which is enhanced by interaction with BANP. Phosphorylated by NUAK1 at Ser-15 and Ser-392; was intially thought to be mediated by STK11/LKB1 but it was later shown that it is indirect and that STK11/LKB1-dependent phosphorylation is probably mediated by downstream NUAK1 . It is unclear whether AMP directly mediates phosphorylation at Ser-15. Phosphorylated on Thr-18 by isoform 1 and isoform 2 of VRK2. Phosphorylation on Thr-18 by isoform 2 of VRK2 results in a reduction in ubiquitination by MDM2 and an increase in acetylation by EP300. Stabilized by CDK5-mediated phosphorylation in response to genotoxic and oxidative stresses at Ser-15, Ser-33 and Ser-46, leading to accumulation of p53/TP53, particularly in the nucleus, thus inducing the transactivation of p53/TP53 target genes. Phosphorylated by DYRK2 at Ser-46 in response to genotoxic stress. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage. Dephosphorylated by PP2A-PPP2R5C holoenzyme at Thr-55. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A.; May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line. Ubiquitinated by MDM2 and SYVN1, which leads to proteasomal degradation . Ubiquitinated by RFWD3, which works in cooperation with MDM2 and may catalyze the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome . Ubiquitinated by MKRN1 at Lys-291 and Lys-292, which leads to proteasomal degradation . Deubiquitinated by USP10, leading to its stabilization . Ubiquitinated by TRIM24, RFFL, RNF34 and RNF125, which leads to proteasomal degradation . Ubiquitination by TOPORS induces degradation . Deubiquitination by USP7, leading to stabilization . Isoform 4 is monoubiquitinated in an MDM2-independent manner . Ubiquitinated by RFWD2, which leads to proteasomal degradation . Ubiquitination and subsequent proteasomal degradation is negatively regulated by CCAR2 . Monomethylated at Lys-372 by SETD7, leading to stabilization and increased transcriptional activation. Monomethylated at Lys-370 by SMYD2, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity. Lys-372 monomethylation prevents interaction with SMYD2 and subsequent monomethylation at Lys-370. Dimethylated at Lys-373 by EHMT1 and EHMT2. Monomethylated at Lys-382 by KMT5A, promoting interaction with L3MBTL1 and leading to repress transcriptional activity. Dimethylation at Lys-370 and Lys-382 diminishes p53 ubiquitination, through stabilizing association with the methyl reader PHF20. Demethylation of dimethylated Lys-370 by KDM1A prevents interaction with TP53BP1 and represses TP53-mediated transcriptional activation.; Sumoylated with SUMO1. Sumoylated at Lys-386 by UBC9.
Cellular Localization Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix.. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2. Translocates to mitochondria upon oxidative stress. Translocates to mitochondria in response to mitomycin C treatment . Isoform 1: Nucleus. Cytoplasm.. Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4.; Isoform 2: Nucleus. Cytoplasm.. Localized mainly in the nucleus with minor staining in the cytoplasm.; Isoform 3: Nucleus. Cytoplasm.. Localized in the nucleus in most cells but found in the cytoplasm in some cells.; Isoform 4: Nucleus. Cytoplasm.. Predominantly nuclear but translocates to the cytoplasm following cell stress.; Isoform 7: Nucleus. Cytoplasm.. Localized mainly in the nucleus with minor staining in the cytoplasm.; Isoform 8: Nucleus. Cytoplasm.. Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli.; Isoform 9: Cytoplasm.
Tissue Specificity Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detec
Sequence and Domain Family The nuclear export signal acts as a transcriptional repression domain. The TADI and TADII motifs (residues 17 to 25 and 48 to 56) correspond both to 9aaTAD motifs which are transactivation domains present in a large number of yeast and animal transcription factors.
Swiss-Prot Key P04637_HUMAN
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