SHOPPING BASKET: 0 item(s) - £0.00  

You have no items in your shopping cart.

Anti-PCNA antibody

[STJ96933] Download PDF Print Data Sheet

* Required Fields

£0.00
Product name Anti-PCNA antibody
Short Description Mouse monoclonal to PCNA.
Description PCNA is a protein encoded by the PCNA gene which is approximately 28,7 kDa. PCNA is localised to the nucleus. It is involved in the telomere C-strand synthesis and trans-lesion synthesis. It is found in the nucleus and is a cofactor of DNA polymerase-delta. It acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is expressed in the liver, lung, bone marrow, muscle and kidney. Mutations in the PCNA gene may result in ataxia-telangiectasia-like disorder. STJ96933 was developed from clone 12D10 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous PCNA protein.
Applications IHC, WB
Dilution range WB 1:5000
IHC 1:200
Specificity The antibody detects endogenous PCNA protein.
Protein Name Proliferating cell nuclear antigen
PCNA
Cyclin
Immunogen Synthetic Peptide
Immunogen Region N-term
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Mouse
Clonality Monoclonal
Clone ID 12D10
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Isotype IgG1
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 5111
Gene Symbol PCNA
Database Links HGNC:8729
OMIM:176740
Alternative Names Proliferating cell nuclear antigen antibody
Cyclin antibody
Function Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways . Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Post-translational Modifications Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA. Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation . Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner . Acetylation disrupts interaction with NUDT15 and promotes degradation . Ubiquitinated . Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase. Methylated on glutamate residues by ARMT1/C6orf211.
Cellular Localization Nucleus. Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage . Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
Swiss-Prot Key P12004_HUMAN
Bad Good
Enter the code in the box below:

Ask us a question

-2
I am interested in this antibody in 0.3mg size, 1mg/mL. Would it be possible to change the storage buffer to PBS and not include sodium azide?
Question by: Guest on 11 Apr 2018 12:40:00
-8
Hello, thank you for your query. If you would like to place a custom order, the product should be at least 1 mg. There will be no additional cost for changing the storage buffer. Please contact us at info@stjohnslabs.com for further information.
Answer by: Customer Support on 11 Apr 2018 12:43:00

Tags:

Use spaces to separate tags. Use single quotes (') for phrases.