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Anti-Phospho-Cofilin (S3) antibody

[STJ90230] Download PDF Print Data Sheet

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Product name Anti-Phospho-Cofilin (S3) antibody
Short Description Rabbit polyclonal to Phospho-Cofilin (S3).
Description Cofilin is a protein encoded by the JAK1 gene which is approximately 133,2 kDa. Cofilin is localised to the endomembrane system and is involved in RET signalling, common cytokine receptor gamma-chain family signalling pathways, Th17 cell differentiation and IL27-mediated signalling events. It phosphorylates STAT proteins and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Cofilin is expressed at higher levels in primary colon tumor than normal colon tissue. Mutations in the JAK1 gene may result in mixed lacrimal gland cancer, t-cell prolymphocytic leukemia and chilblain lupus. The antibody STJ90230 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous Cofilin protein which only binds about S3 when S3 is phosphorylated.
Applications ELISA, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Specificity Phospho-Cofilin (S3) Polyclonal Antibody detects endogenous levels of Cofilin protein only when phosphorylated at S3.
Protein Name Cofilin-1
18 kDa phosphoprotein
p18
Cofilin, non-muscle isoform
Immunogen Synthesized peptide derived from human Cofilin around the phosphorylation site of S3.
Immunogen Region 10-90 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1072
Gene Symbol JAK1
Molecular Weight 19 kDa
Database Links HGNC:1874
OMIM:601442
Alternative Names Phospho-Cofilin (S3)
Cofilin-1 antibody
18 kDa phosphoprotein antibody
p18 antibody
Cofilin, non-muscle isoform antibodyA antibodyB antibody
Function Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway . Kinase partner for the interleukin (IL)-2 receptor .
Post-translational Modifications Autophosphorylated . Phosphorylated on tyrosine residues in response to interferon gamma signaling . Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling . Ubiquitinated by RNF125; leading to its degradation by the proteasome.
Cellular Localization Endomembrane system. Peripheral membrane protein. Wholly intracellular, possibly membrane associated.
Tissue Specificity Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
Sequence and Domain Family Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1. The FERM domain mediates interaction with JAKMIP1.
Swiss-Prot Key P23458_HUMAN
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