Anti-Phospho-eIF2α (S51) antibody

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Product name Anti-Phospho-eIF2α (S51) antibody
Short Description Rabbit polyclonal to Phospho-eIF2α (S51).
Description eIF2α is a protein encoded by the EIF2S1 gene which is approximately 36,1 kDa. eIF2α is localised to the cytoplasmic granule and is involved in viral mRNA translation, influenza A, translation factors and regulation of lipid metabolism. It functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. eIF2α is expressed in the liver, nervous system, lung, blood and kidney. Mutations in the EIF2S1 gene result in Wolcott-Rallison syndrome. The antibody STJ90253 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous eIF2α protein which only binds about S51 when S51 is phosphorylated.
Applications ELISA, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Specificity Phospho-eIF2α (S51) Polyclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51.
Protein Name Eukaryotic translation initiation factor 2 subunit 1
Eukaryotic translation initiation factor 2 subunit alpha
eIF-2-alpha
eIF-2A
eIF-2alpha
Immunogen Synthesized peptide derived from human eIF2α around the phosphorylation site of S51.
Immunogen Region 30-110 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IF WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 1965
Gene Symbol EIF2S1
Molecular Weight 36 kDa
Database Links HGNC:3265
OMIM:603907
Alternative Names Phospho-eIF2a (S51)
Eukaryotic translation initiation factor 2 subunit 1 antibody
Eukaryotic translation initiation factor 2 subunit alpha antibody
eIF-2-alpha antibody
eIF-2A antibody
eIF-2alpha antibody
EIF2A antibody
Function Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Post-translational Modifications Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation . Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation .
Cellular Localization Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules .
Swiss-Prot Key P05198_HUMAN
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