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Anti-Phospho-JNK1/2/3 (T183/Y185) antibody

[STJ90317] Download PDF Print Data Sheet

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Product name Anti-Phospho-JNK1/2/3 (T183/Y185) antibody
Short Description Rabbit polyclonal to Phospho-JNK1/2/3 (T183/Y185).
Description JNK1/2/3 is a protein encoded by the MAPK8 gene which is approximately 48,2 kDa. JNK1/2/3 is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling, the IL-2 pathway, TNFR1 pathway, toll-like receptor signalling pathways and the 4-1BB pathway. It acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, therefore mediating immediate-early gene expression in response to cell stimuli. JNK1/2/3 is expressed in the nervous system, liver, blood, heart and lung. Mutations in the MAPK8 gene may result in fatty liver disease and renal fibrosis. STJ90317 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous JNK1/2/3 protein which only binds about T183/Y185 when T183/Y185 is phosphorylated.
Applications ELISA, WB
Dilution range WB 1:500-1:2000
ELISA 1:10000
Specificity Phospho-JNK1/2/3 (T183/Y185) Polyclonal Antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at T183/Y185.
Protein Name Mitogen-activated protein kinase 8
MAP kinase 8
MAPK 8
JNK-46
Stress-activated protein kinase 1c
SAPK1c
Stress-activated protein kinase JNK1
c-Jun N-terminal kinase 1
Immunogen Synthesized peptide derived from human JNK1/2/3 around the phosphorylation site of T183/Y185.
Immunogen Region 120-200 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Chicken, Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 5599
Gene Symbol MAPK8
Molecular Weight 48 kDa
Database Links HGNC:6881
OMIM:601158
Alternative Names Phospho-JNK1/2/3 (T183/Y185)
Mitogen-activated protein kinase 8 antibody
MAP kinase 8 antibody
MAPK 8 antibody
JNK-46 antibody
Stress-activated protein kinase 1c antibody
SAPK1c antibody
Stress-activated protein kinase JNK1 antibody
c-Jun N-terminal kinase 1 antibody
JNK1 antibody
PRKM8 antibody
SAPK1 antibody
SAPK1C antibody
Function Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock . Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity . JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
Post-translational Modifications Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2.
Cellular Localization Cytoplasm Nucleus
Sequence and Domain Family The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Swiss-Prot Key P45983_HUMAN
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