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Anti-Phospho-p70 S6 kinase α (S447) antibody

[STJ90374] Download PDF Print Data Sheet
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Product name Anti-Phospho-p70 S6 kinase α (S447) antibody
Short Description Rabbit polyclonal to Phospho-p70 S6 kinase α (S447).
Description Phospho-p70 S6 kinase alpha is a protein encoded by the RPS6KB1 gene which is approximately 59,2 kDa. Phospho-p70 S6 kinase alpha is localised to the mitochondrion and cell junctions. It is involved in pathways such as the common cytokine receptor gamma-c family signalling pathway, regulation of lipid metabolism insulin signalling-generic cascades, RET signalling and mTOR signalling. This protein falls under the ribosomal S6 kinase family of serine/threonine kinases. It responds to mTOR signalling and is activated in response to growth factors and nutrients to promote protein synthesis, cell growth, cell cycle progression and cell proliferation. It regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Phospho-p70 S6 kinase alpha is expressed in the cells of the eye, nervous system, muscle, heart and liver. Mutations in the RPS6KB1 gene result in diseases such as tuberous sclerosis which manifests as hamartoma formation in multiple organ systems and glioblastoma multiforme which is a central nervous system tumour of grade IV histological malignancy. STJ90374 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The synthesized peptide derived from human p70 S6 kinase alpha around the phosphorylation site of S447. This primary antibody specifically binds to endogenous p70 S6 kinase α protein which only binds about S447 when S447 is phosphorylated.
Applications ELISA, IHC-p, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Specificity Phospho-p70 S6 kinase α (S447) Polyclonal Antibody detects endogenous levels of p70 S6 kinase α protein only when phosphorylated at S447.
Protein Name Ribosomal protein S6 kinase beta-1
S6K-beta-1
S6K1
70 kDa ribosomal protein S6 kinase 1
P70S6K1
p70-S6K 1
Ribosomal protein S6 kinase I
Serine/threonine-protein kinase 14A
p70 ribosomal S6 kinase alpha
p70 S6 kinase alpha
p70 S6K-alpha
p70 S6KA
Immunogen Synthesized peptide derived from human p70 S6 kinase α around the phosphorylation site of S447.
Immunogen Region 390-470 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Mouse, Rat, Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 6198
Gene Symbol RPS6KB1
Molecular Weight 70 kDa
Database Links HGNC:10436
OMIM:608938
Alternative Names Phospho-p70 S6 kinase a (S447)
Ribosomal protein S6 kinase beta-1 antibody
S6K-beta-1 antibody
S6K1 antibody
70 kDa ribosomal protein S6 kinase 1 antibody
P70S6K1 antibody
p70-S6K 1 antibody
Ribosomal protein S6 kinase I antibody
Serine/threonine-protein kinase 14A antibody
p70 ribosomal S6 kinase alpha antibody
p70 S6 kinase alpha antibody
p70 S6K-alpha antibody
p70 S6KA antibody
STK14A antibody
Function Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR.
Post-translational Modifications Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism . Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.
Cellular Localization Cell junction, synapse, synaptosome Mitochondrion outer membrane. Mitochondrion.. Colocalizes with URI1 at mitochondrion.; Isoform Alpha I: Nucleus. Cytoplasm.; Isoform Alpha II: Cytoplasm.
Tissue Specificity Widely expressed.
Sequence and Domain Family The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.; The TOS (TOR signaling) motif is essential for activation by mTORC1.
Swiss-Prot Key P23443_HUMAN
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