Anti-Phospho-RB1-(S807) Antibody

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Product name Anti-Phospho-RB1-(S807) Antibody
Short Description Rabbit monoclonal against Phospho-RB1-(S807)
Description The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Applications IF, IHC, WB
Dilution range WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Protein Name Retinoblastoma-associated protein
p105-Rb
pRb
Rb
pp110
Immunogen A phospho specific peptide corresponding to residues surrounding S807 of human RB1
Storage Instruction Store at -20C. Avoid freeze / thaw cycles.
Note For Research Use Only (RUO).
Validated Application WB IHC IF
Host Rabbit
Clonality Monoclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Gene ID 5925
Gene Symbol RB1
Molecular Weight 106.159 kDa
Database Links HGNC:9884
OMIM:109800
Reactome:R-HSA-113501
Alternative Names Retinoblastoma-associated protein
p105-Rb
pRb
Rb
pp110
Function Key regulator of entry into cell division that acts as a tumor suppressor, Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C, Acts as a transcription repressor of E2F1 target genes, The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest, Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation, Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression, Controls histone H4 'Lys-20' trimethylation, Inhibits the intrinsic kinase activity of TAF1, Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter, In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex, Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex , In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity,
Post-translational Modifications Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth, Dephosphorylated at the late M phase, SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb, Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes, Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation, CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition, Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis ,
Cellular Localization Nucleus
Tissue Specificity Expressed in the retina
Swiss-Prot Key P06400
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