Anti-Phospho-RIP2 (S176) antibody

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Product name Anti-Phospho-RIP2 (S176) antibody
Short Description Rabbit polyclonal to Phospho-RIP2 (S176).
Description RIP2 is a protein encoded by the RIPK2 gene which is approximately 61,2 kDa. RIP2 is localised to the cytoplasm. It is involved in activated TLR4 signalling, p75 NTR receptor-mediated signalling, toll-like receptor signalling pathways and cytokine signalling in immune system. It is a serine/threonine/tyrosine kinase that contains a C-terminal caspase activation and recruitment domain and plays an essential role in modulation of innate and adaptive immune responses. RIP2 is expressed in the heart, brain, placenta, lung, peripheral blood leukocytes, spleen and kidney. Mutations in the RIPK2 gene may result in x-linked lymphoproliferative syndrome and human granulocytic anaplasmosis. STJ90957 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous RIP2 protein which only binds about S176 when S176 is phosphorylated.
Applications ELISA, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Specificity Phospho-RIP2 (S176) Polyclonal Antibody detects endogenous levels of RIP2 protein only when phosphorylated at S176.
Protein Name Receptor-interacting serine/threonine-protein kinase 2
CARD-containing interleukin-1 beta-converting enzyme-associated kinase
CARD-containing IL-1 beta ICE-kinase
RIP-like-interacting CLARP kinase
Receptor-interacting protein 2
Immunogen Synthesized peptide derived from human RIP2 around the phosphorylation site of S176.
Immunogen Region 120-200 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IHC WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 8767
Gene Symbol RIPK2
Molecular Weight 70 kDa
Database Links HGNC:10020
OMIM:603455
Alternative Names Receptor-interacting serine/threonine-protein kinase 2
CARD-containing interleukin-1 beta-converting enzyme-associated kinase
CARD-containing IL-1 beta ICE-kinase
RIP-like-interacting CLARP kinase
Receptor-interacting protein 2
Function Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
Post-translational Modifications Autophosphorylated. Autophosphorylation at Tyr-474 is necessary for effective NOD2 signaling. Phosphorylated. Phosphorylation at Tyr-381 by Src kinase CSK occurs in a ARHGEF2-dependent manner and is required for NOD2-dependent innate immune activation. Ubiquitinated on Lys-209; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH. Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B. Also undergoes 'Met-1'-linked polyubiquitination; the head-to-tail linear polyubiquitination is mediated by the LUBAC complex in response to NOD2 stimulation. Linear polyubiquitination is restricted by FAM105B/otulin, probably to limit NOD2-dependent proinflammatory signaling activation of NF-kappa-B.
Cellular Localization Cytoplasm
Tissue Specificity Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
Sequence and Domain Family Contains an N-terminal kinase domain and a C-terminal caspase activation and recruitment domain (CARD) that mediates the recruitment of CARD-containing proteins.
Swiss-Prot Key O43353_HUMAN
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