Anti-Venus antibody

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Product name Anti-Venus antibody
Short Description Goat polyclonal against Venus
Description Goat polyclonal antibody to Venus. Venus is a genetic mutant of green fluorescent protein (GFP), derived from Aequorea victoria. Its excitation/emission peaks are 515/528 nm respectively and is often used as a donor-acceptor pair by researchers in FRET based co-localization studies.
Applications IEM, IF, IHC, WB
Dilution range Western blot 1:500-1:2,000 Immunofluorescence 1:50-1:1,000 Immunohistochemistry (paraffin) 1:50-1:1,000 Immunohistochemistry (frozen) 1:50-1:1,000
Specificity In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody also recognizes Venus/GFP and does not cross-react to mCherry/red fluorescent proteins.
Immunogen Purified recombinant GFP peptide from the jellyfish aequorea victoria produced in E. coli.
Storage Instruction Store at -20°C, and avoid repeated freeze-thaw cycles.
Note For research use only (RUO).
Validated Application WB IF IHC
Host Goat
Clonality Polyclonal
Reactivity EGFP, GFP, Venus, YFP
Conjugation Unconjugated
Concentration 3 mg/mL
Purification This antibody is epitope-affinity purified from goat antiserum.
Isotype IgG
Formulation PBS, 20% glycerol and 0.05% sodium azide.
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