Polyclonal Antibodies

Polyclonal Antibodies


Polyclonal antibodies (pAbs) are typically affinity purified from the antiserum of an immunised animal. This process leads to the production of high titer and high affinity polyclonal antibodies specifically against the antigen of interest.


The use of polyclonal antibodies (PAbs) over monoclonal antibodies has its advantages and the technical skill needed to develop polyclonal antibodies is not as demanding. They're inexpensive to make and can be generated fairly quickly, but usually in batches over several months. They are heterogeneous, which allows them to bind to a wide range of epitopes on a given antigen. And, because polyclonal antibodies are produced from a large number of B cell clones, they're more likely to successfully bind to a specific antigen. They also remain relatively stable in different environments, such as a change in pH or salt concentration, which allows them to be more suitable in certain procedures. Depending on the amount needed polyclonal antibodies can be developed in large quantities in relation to the size of the animal used

St John's Laboratory provide a wide range of polyclonal antibodies: rabbit antibodies, goat antibodies, chicken antibodies, sheep antibodies, rat antibodies, and guinea pig antibodies.

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  • Anti-Factor VIII antibody [STJ93018]

    Western Blot (WB) analysis of specific cells using Factor VIII polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse
    Applications: ELISA, IHC, WB
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  • Anti-TG beta antibody [STJ95997]

    Western blot (WB) analysis of TGFbeta1 polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC, WB
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  • Anti-ZO1 antibody [STJ140055]

    Immunofluorescence was carried out on primary RPE cells, using Anti-ZO1 polyclonal antibody at a 1:100 dilution.
    Clonality: Polyclonal
    Host: Goat
    Reactivity: Avian, Bovine, Canine, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Simian, Swine
    Applications: IF, IHC, WB
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  • Anti-ENT1 antibody [STJ96396]

    Western Blot (WB) analysis of extracts from Jurkat, K562, NIH-3T3 cells, using ENT1 polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, WB
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  • Anti-Phospho-Akt (S473) antibody [STJ90166]

    Western blot (WB) analysis of AKT (phospho Ser473) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IHC, WB
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  • Anti-beta-actin antibody [STJ97089]

    Western Blot (WB) analysis of HepG2 (1), Rat liver (2), Mouse Kidney (3), Rabbit testic (4), Sheep lung (5), 293T (6), diluted at 1:5000.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Avian, Human, Mouse, Rabbit, Rat, Sheep, Simian, Xenopus
    Applications: IHC, WB
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  • Anti-Integrin alpha antibody [STJ97284]

    Western Blot (WB) analysis of HeLa cells using Integrin alpha5 polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC, WB
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  • Anti-beta III Tubulin antibody [STJ97123]

    Western Blot (WB) analysis of 1) HeLa, 2) Mouse Brain, 3) Rat Brain tissue, diluted at 1:5000.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: FC, IF, WB
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  • Anti-Histone H3 (Acetyl K9) antibody [STJ90121]

    Western blot (WB) analysis of Histone H3 (Acetyl Lys9) polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC, WB
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  • Anti-mTOR antibody [STJ94280]

    Western Blot (WB) analysis of HY926 cells using Anti-mTOR polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, IF, IHC, WB
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  • Anti-CAR antibody [STJ91996]

    Western Blot (WB) analysis of specific cells using CAR polyclonal antibody.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: Human, Mouse, Rat
    Applications: ELISA, WB
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  • Anti-Pan myristoylation antibody [STJ98666]

    Western Blot (WB) analysis of KB, Mouse Lung cells using Antibody diluted at 1:1000.
    Clonality: Polyclonal
    Host: Rabbit
    Reactivity: All
    Applications: ELISA, WB
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