Beta Actin

Beta Actin – What’s so special about it? Out of all the thousands and thousands of known proteins that exist within the human genome, actin is the most abundant

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IHC Guaranteed Antibodies

Discover range of IHC Guaranteed Products

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Antibody Validation Project

Join our Antibody Validation Project and trial free samples

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Western Blot Membrane Stripping

 

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Brief History of Antibodies

 

 

From Vaccines to monoclonals

Over the past two centuries research and treatment regarding the knowledge and applications of antibodies has come a long way from its early discoveries and basic understanding...

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St John's Laboratory - Blog

Antibody validation and research tips

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    Join the Antibody Validation Project to Receive Free ACTB Trial Size Antibodies

      Join the Antibody Validation Project to Receive Free ABCG2 Trial Size Antibodies Antibody Validation Project - Check out our reviewed ABCG2 antibodies Model Name Host Clonality Rating Application Tested STJ97253 Anti-ABCG2 antibody Rabbit Polyclonal 5 WB   We also have other ABCG2 antibodies available in the Antibody Validation Project...

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    The benefits of choosing rabbit monoclonal antibodies over regular monoclonal or polyclonal antibodies

    Introduction When it comes to identifying proteins in research or developing therapeutics for clinical use, antibodies have become uniquely placed as the most suitable option. Ever since Georges J.F. Köhler and César Milstein first described the hybridoma technique for the production of monoclonal antibodies in 19741,2, antibodies have become indispensable...

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    Choosing Between a Sandwich and Competitive ELISA

    1. What is an ELISA? Enzyme Linked Immunosorbent Assay (ELISA) is a simple, quick, sensitive and reliable analytical technique used for detecting and quantifying protein biomarkers in immunoassays1. ELISA’s have been used for decades as a diagnostic tool and remains a gold standard for pharmacological and clinical diagnostics2. 2. How...

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    How to get the most out of your ELISA

    How to get the most out of your ELISA ELISA (enzyme-linked immunosorbent assay) is a common test for detecting and quantifying proteins, particularly antibodies. The ability of ELISA to quantify proteins is the reason you might choose this technique over qualitative methods, such as western blotting. The (very) basic steps...

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    Using Rf value to calculate molecular weight

    Using Rf value to calculate molecular weight In a previous post, we looked at how to calculate the Rf value of a protein from a western blot. The formula is: Rf = Migration distance of the protein Migration distance of the dye front So why do we need the Rf...
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