St Johns Labs

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  • Antibody Validation Project

    Antibody Validation Project Did you know that around 98% of bio-scientists will use antibodies at least once in their career?! So most of the researchers in your lab will have some experience with antibodies. Take a look around – that guy using the PCR machine who you’ve never spoken to...
  • Monoclonal antibodies VS polyclonal antibodies - Which to choose?

    If you’re reading this then hopefully you have an interest for antibodies or you are contemplating which of the two types you ought to use within your research. To begin, let’s cover a few basics. Monoclonal antibodies – these singular types of antibody are produced by harvesting purified cell lines...
  • All you need to know when choosing a Gradient Gel

    Why use a Gradient gel? If you’ve found yourself contemplating which type of polyacrylamide gel you wish to use for your western blotting protocol its worth having a read and learning about Gradient gels. Gradient gels are a great form of polyacrylamide gel to use during a Western Blot, especially...
  • Genetic strategies for antibody validation

    Researchers invest precious resources in studies that rely on the specificity of their chosen antibody. Often, the success or failure of a project hinges on the specificity of an antibody: whether it consistently recognizes its target with little or no cross-reactivity. Clearly, researchers need to feel confident in the performance...
  • The Foundations of Antibody Validation

    For many life science researchers, antibodies are an essential part of the laboratory toolkit. They are the foundation of many important scientific discoveries, as well as the building blocks of innumerable publications. Antibodies are most commonly employed in western blot assays (immunoblotting), and are also regularly used in immunohistochemistry and...
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