Beta Actin

Beta Actin – What’s so special about it? Out of all the thousands and thousands of known proteins that exist within the human genome, actin is the most abundant

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IHC Guaranteed Antibodies

Discover range of IHC Guaranteed Products

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Antibody Validation Project

Join our Antibody Validation Project and trial free samples

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Western Blot Membrane Stripping

 

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Brief History of Antibodies

 

 

From Vaccines to monoclonals

Over the past two centuries research and treatment regarding the knowledge and applications of antibodies has come a long way from its early discoveries and basic understanding...

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St John's Laboratory - Blog

    Recent Posts

    All you need to know when choosing a Gradient Gel

    All you need to know when choosing a Gradient Gel Why use a Gradient gel? If you’ve found yourself contemplating which type of polyacrylamide gel you wish to use for your western blotting protocol its worth having a read and learning about Gradient gels. Gradient gels are a great form...

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    Genetic strategies for antibody validation

    Genetic strategies for antibody validation   Researchers invest precious resources in studies that rely on the specificity of their chosen antibody. Often, the success or failure of a project hinges on the specificity of an antibody: whether it consistently recognises its target with little or no cross-reactivity. Clearly, researchers need...

    Recent Posts

    The Foundations of Antibody Validation

    The Foundations of Antibody Validation For many life science researchers, antibodies are an essential part of the laboratory toolkit. They are the foundation of many important scientific discoveries, as well as the building blocks of innumerable publications. Antibodies are most commonly employed in western blot assays (immunoblotting), and are also...

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    Choosing BSA vs Non-Fat Milk

    Which is best? BSA vs Non-Fat Milk So, you are about to carry out a Western Blot. As a researcher you may be asking yourself one of the most common, and appropriate questions there is in regards to setting up your experiment. “Which blocking buffer/blocking agent do I use?” You’ve...

    Recent Posts

    Western Blot Membrane Stripping

    Stripping for Science! (…Your membrane of course) The process of western blot membrane stripping is a procedure used to remove both the primary and secondary antibodies bound to proteins present on your transfer membrane. Why would you strip your western blot membrane? Western blot stripping has the advantages of saving...