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    How to calculate the Rf value of a protein from western blot

    How to calculate the Rf value of a protein from western blot Western blot is a common tool for identifying, quantifying and determining the size of specific proteins. Essentially, western blotting allows a mixture of proteins to be separated using gel electrophoresis. The proteins are then transferred from the gel...

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    Tips For Optimal Flow Cytometry

    Tips For Optimal Flow Cytometry Flow cytometry is a widely utilised laser-based technique which lends itself to the analysis of various physical and chemical characteristics in a particular cell population. The method has grown to be a popular technique within the realm of antibody research through its proven use in...

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    Immunofluorescence Troubleshooting

    Immunofluorescence Troubleshooting As most researchers know, getting a technique to work in the way it’s supposed to can be a matter of trial and error. Immunofluorescence (IF), a technique that uses antibodies labelled with a fluorescent dye to detect a target antigen, has increased in popularity over the last few...

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    The Bradford Protein Assay Tips - Preparing For Western Blotting

    The Bradford Protein Assay is a simple spectroscopic technique which is used in laboratory research to measure the total concentration of protein in a particular sample. The principle of the procedure revolves around the concept that the maximum absorbance of acidic Coomassie Brilliant Blue G-250 alters when protein binding occurs...

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    Immunohistochemistry - getting the most out of your IHC protocol

    Immunohistochemistry - getting the most out of your IHC protocol The application of immunohistochemistry provides a visual opportunity to interpret tissue integrity, and allows for monitoring of the way in which protein expression is distributed across them. In previous years the application mainly visualised proteins using the chromogenic method, but...
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