Tips

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    Antibodies in immunofluorescence: top tips

    Antibodies in immunofluorescence: top tips If you work with antibodies, the chances are that you’re familiar with immunofluorescence (IF), or at least the application term. This common laboratory technique uses antibodies labelled with a fluorescent dye to detect a target antigen. Popular due to its reliability and relative simplicity, IF...

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    Top tips for optimum SDS-PAGE results

    Top tips for optimum SDS-PAGE results The process of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique in biochemistry, molecular biology and biotechnology. The method is commonly combined with antibody-based detection in Western blotting procedures and can be used in antibody application to differentiate proteins from...

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    Monoclonal antibodies VS polyclonal antibodies - Which to choose?

    Monoclonal antibodies VS polyclonal antibodies - Which to choose? If you’re reading this then hopefully you have an interest for antibodies or you are contemplating which of the two types you ought to use within your research. To begin, let’s cover a few basics. Monoclonal antibodies – these singular types...

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    All you need to know when choosing a Gradient Gel

    All you need to know when choosing a Gradient Gel Why use a Gradient gel? If you’ve found yourself contemplating which type of polyacrylamide gel you wish to use for your western blotting protocol its worth having a read and learning about Gradient gels. Gradient gels are a great form...

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    Choosing BSA vs Non-Fat Milk

    Which is best? BSA vs Non-Fat Milk So, you are about to carry out a Western Blot. As a researcher you may be asking yourself one of the most common, and appropriate questions there is in regards to setting up your experiment. “Which blocking buffer/blocking agent do I use?” You’ve...
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