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    Immunohistochemistry - getting the most out of your IHC protocol

    Immunohistochemistry - getting the most out of your IHC protocol The application of immunohistochemistry provides a visual opportunity to interpret tissue integrity, and allows for monitoring of the way in which protein expression is distributed across them. In previous years the application mainly visualised proteins using the chromogenic method, but...

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    Antibodies in immunofluorescence: top tips

    Antibodies in immunofluorescence: top tips If you work with antibodies, the chances are that you’re familiar with immunofluorescence (IF), or at least the application term. This common laboratory technique uses antibodies labelled with a fluorescent dye to detect a target antigen. Popular due to its reliability and relative simplicity, IF...

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    Top tips for optimum SDS-PAGE results

    Top tips for optimum SDS-PAGE results The process of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique in biochemistry, molecular biology and biotechnology. The method is commonly combined with antibody-based detection in Western blotting procedures and can be used in antibody application to differentiate proteins from...

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    Monoclonal antibodies VS polyclonal antibodies - Which to choose?

    Monoclonal antibodies VS polyclonal antibodies - Which to choose? If you’re reading this then hopefully you have an interest for antibodies or you are contemplating which of the two types you ought to use within your research. To begin, let’s cover a few basics. Monoclonal antibodies – these singular types...

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    All you need to know when choosing a Gradient Gel

    All you need to know when choosing a Gradient Gel Why use a gradient gel? If you’ve found yourself contemplating which type of polyacrylamide gel you wish to use for your western blotting protocol its worth having a read and learning about gradient gels. Gradient gels are a great form...