Western Blot

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    How to calculate the Rf value of a protein from western blot

    How to calculate the Rf value of a protein from western blot Western blot is a common tool for identifying, quantifying and determining the size of specific proteins. Essentially, western blotting allows a mixture of proteins to be separated using gel electrophoresis. The proteins are then transferred from the gel...

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    Tips for Successful Western Blotting

    Tips for Successful Western Blotting Western blotting is a powerful tool for studying proteins. As a popular technique, encountered by everyone from first-year students to experienced researchers, western blotting has a number of functions. In general, it is useful for identifying, semi-quantifying (it can show relative protein levels, but not...

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    The Bradford Protein Assay Tips - Preparing For Western Blotting

    The Bradford Protein Assay is a simple spectroscopic technique which is used in laboratory research to measure the total concentration of protein in a particular sample. The principle of the procedure revolves around the concept that the maximum absorbance of acidic Coomassie Brilliant Blue G-250 alters when protein binding occurs...

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    Top tips for optimum SDS-PAGE results

    Top tips for optimum SDS-PAGE results The process of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique in biochemistry, molecular biology and biotechnology. The method is commonly combined with antibody-based detection in Western blotting procedures and can be used in antibody application to differentiate proteins from...