Anti-Connexin 43 antibody




 
Description
Rabbit polyclonal to Connexin 43.
 


Product Information

Model STJ92411
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC, WB
Immunogen Synthesized peptide derived from human Connexin 43
Gene ID 2697
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Specificity Connexin 43 Polyclonal Antibody detects endogenous levels of Connexin 43 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Note For Research Use Only (RUO).
Protein Name Gap junction alpha-1 protein
Connexin-43
Cx43
Gap junction 43 kDa heart protein
Clonality Polyclonal
Conjugation Unconjugated
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration 1 mg/ml
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Database Links HGNC:4274
OMIM:104100
Alternative Names Gap junction alpha-1 protein
Connexin-43
Cx43
Gap junction 43 kDa heart protein
Function Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract . May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles .
Cellular Localization Cell membrane Cell junction, gap junction Endoplasmic reticulum. Localizes at the intercalated disk (ICD) in cardiomyocytes and the proper localization at ICD is dependent on TMEM65.
Post-translational Modifications Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity . Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation . Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2. S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.


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