Anti-LC3A antibody




 
Description
Mouse monoclonal against LC3A
 


Product Information

Model STJ97755
Host Mouse
Reactivity Mouse, Rat, Human
Applications IF, IHC-p, WB
Immunogen synthetic peptide derived from LC3A
Gene ID 84557
Dilution range WB 1:1000 - 2000
IHC 1:100 - 200
IF 1:200
Specificity LC3A protein detects endogenous levels of LC3A
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Clone ID 5G10
Note For Research Use Only (RUO).
Protein Name Microtubule-Associated Proteins 1a/1b Light Chain 3a
Autophagy-Related Protein Lc3 A
Autophagy-Related Ubiquitin-Like Modifier Lc3 A
Map1 Light Chain 3-Like Protein 1
Map1a/Map1b Light Chain 3 A
Map1a/Map1b Lc3 A
Microtubule-Associated Protein 1 Light Chain 3 Alpha
Clonality Monoclonal
Conjugation Unconjugated
Isotype
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration 1 mg/ml
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Database Links HGNC:6838
OMIM:601242
Alternative Names Anti-Microtubule-Associated Proteins 1a/1b Light Chain 3a antibody
Anti-Autophagy-Related Protein Lc3 A antibody
Anti-Autophagy-Related Ubiquitin-Like Modifier Lc3 A antibody
Anti-Map1 Light Chain 3-Like Protein 1 antibody
Anti-Map1a/Map1b Light Chain 3 A antibody
Anti-Map1a/Map1b Lc3 A antibody
Anti-Microtubule-Associated Protein 1 Light Chain 3 Alpha antibody
Anti-MAP1LC3A antibody
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, paticipates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover.
Cellular Localization Cytoplasm
Cytoskeleton
Endomembrane System
Lipid-Anchor
Cytoplasmic Vesicle
Autophagosome Membrane
Autophagosome
Lc3-Ii Binds To The Autophagic Membranes
Post-translational Modifications The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.; The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.; Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.


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