Human Toll-Like Receptor 4 ELISA kit

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Product name Human Toll-Like Receptor 4 ELISA kit
Short Description Human ELISA Kit to TLR4.
Description The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TLR4 in Human serum, plasma and other biological fluids.
Applications ELISA
Dilution range 0.313--20ng/mL
Protein Name TLR4
Storage Instruction Store at 4°C for 6 months.
Note FOR RESEARCH USE ONLY (RUO) .
Validated Application ELISA
Reactivity Human
Gene ID 7099
Gene Symbol TLR4
Molecular Weight 95.7 kDa
Database Links HGNC:11850
OMIM:603030
Reactome:R-HSA-1236974
Alternative Names Toll-Like Receptor 4, Htoll, Cd Antigen Cd284
Function Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) . Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni (2+) . Responses triggered by Ni (2+) require non-conserved histidines and are, therefore, species-specific. Both M.tuberculosis HSP70 (dnaK) and HSP65 (groEL-2) act via this protein to stimulate NF-kappa-B expression. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL-or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL (-) ) and mediates the cytokine release induced by LDL (-) . Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via TLR2, but also partially via this receptor.
Post-translational Modifications N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.; Phosphorylated on tyrosine residues by LYN after binding lipopolysaccharide.
Cellular Localization Cell Membrane, Single-Pass Type I Membrane Protein, Early Endosome, Cell Projection, Ruffle, Note=Upon Complex Formation With Cd36 And Tlr6, Internalized Through Dynamin-Dependent Endocytosis (Pubmed:20037584) , Colocalizes With Rftn1 At Cell Membrane And Then Together With Rftn1 Moves To Endosomes, Upon Lipopolysaccharide Stimulation
Tissue Specificity Serum, Plasma, Biological Fluids
Swiss-Prot Key TLR4_HUMAN
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