Human Adiponectin ELISA kit

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Product name Human Adiponectin ELISA kit
Short Description Human ELISA Kit to ADP/Acrp30.
Description The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ADP/Acrp30 in Human serum, plasma and other biological fluids.
Applications ELISA
Dilution range 0.781--50ng/mL
Protein Name ADP/Acrp30
Storage Instruction Store at 4°C for 6 months.
Note FOR RESEARCH USE ONLY (RUO) .
Validated Application ELISA
Reactivity Human
Gene ID 9370
Gene Symbol ADIPOQ
Molecular Weight 26.4 kDa
Database Links HGNC:13633
OMIM:125853
Reactome:R-HSA-163680
Alternative Names Adiponectin, 30 Kda Adipocyte Complement-Related Protein, Adipocyte Complement-Related 30 Kda Protein, Acrp30, Adipocyte-C1q And Collagen Domain-Containing Protein, Adipose Most Abundant Gene Transcript 1 Protein, Apm-1, Gelatin-Binding Protein
Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Post-translational Modifications HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagen-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.; O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.; Succination of Cys-36 by the Krebs cycle intermediate fumarate, which leads to S- (2-succinyl) cysteine residues, inhibits polymerization and secretion of adiponectin. Adiponectin is a major target for succination in both adipocytes and adipose tissue of diabetic mammals. It was proposed that succination of proteins is a biomarker of mitochondrial stress and accumulation of Krebs cycle intermediates in adipose tissue in diabetes and that succination of adiponectin may contribute to the decrease in plasma adiponectin in diabetes.
Cellular Localization Secreted
Tissue Specificity Serum, Plasma, Biological Fluids
Swiss-Prot Key ADIPO_HUMAN
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