• Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-BCL2L11-Ser59 antibody (31-80 aa) (STJ91225)

SKU:
STJ91225

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Bcl-2-like protein 11-Ser59 (31-80 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCL2L11
Gene ID: 10018
Uniprot ID: B2L11_HUMAN
Immunogen Region: 31-80 aa
Specificity: Phospho-Bim (S59) Polyclonal Antibody detects endogenous levels of Bim protein only when phosphorylated at S59.
Immunogen: The antiserum was produced against synthesized peptide derived from the human BIM around the phosphorylation site of Ser59 at the amino acid range 31-80
Post Translational Modifications Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation. Deubiquitination catalyzed by USP27X stabilizes the protein. Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein.
Function Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.
Protein Name Bcl-2-Like Protein 11
Bcl2-L-11
Bcl2-Interacting Mediator Of Cell Death
Database Links Reactome: R-HSA-111446
Reactome: R-HSA-111453
Reactome: R-HSA-193648
Reactome: R-HSA-6802952
Reactome: R-HSA-8862803
Reactome: R-HSA-8952158
Reactome: R-HSA-9607240
Reactome: R-HSA-9614657
Cellular Localisation Endomembrane System
Peripheral Membrane Protein
Associated With Intracytoplasmic Membranes
Isoform Bimel: Mitochondrion
Translocates From Microtubules To Mitochondria On Loss Of Cell Adherence
Isoform Biml: Mitochondrion
Isoform Bims: Mitochondrion
Isoform Bim-Alpha1: Mitochondrion
Alternative Antibody Names Anti-Bcl-2-Like Protein 11 antibody
Anti-Bcl2-L-11 antibody
Anti-Bcl2-Interacting Mediator Of Cell Death antibody
Anti-BCL2L11 antibody
Anti-BIM antibody

Information sourced from Uniprot.org

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